BAIT

PAIP1

PAIP-1
polyadenylate binding protein-interacting protein 1
GO Process (1)
GO Function (2)
GO Component (0)

Gene Ontology Biological Process

Gene Ontology Molecular Function

Mus musculus
PREY

EIF3A

A830012B05Rik, Csma, Eif3, Eif3s10, mKIAA0139
eukaryotic translation initiation factor 3, subunit A
Mus musculus

Affinity Capture-Western

An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins.

Publication

Control of Paip1-eukayrotic translation initiation factor 3 interaction by amino acids through S6 kinase.

Martineau Y, Wang X, Alain T, Petroulakis E, Shahbazian D, Fabre B, Bousquet-Dubouch MP, Monsarrat B, Pyronnet S, Sonenberg N

The simultaneous interaction of poly(A)-binding protein (PABP) with eukaryotic translation initiation factor 4G (eIF4G) and the mRNA 3' poly(A) tail promotes translation initiation. We previously showed that the interaction of PABP-interacting protein 1 (Paip1) with PABP and eukaryotic translation initiation factor 3 (eIF3; via the eIF3g subunit) further stimulates translation. Here, we demonstrate that the interaction of eIF3 with Paip1 ... [more]

Mol. Cell. Biol. Mar. 01, 2014; 34(6);1046-53 [Pubmed: 24396066]

Throughput

  • Low Throughput

Curated By

  • BioGRID