A genetic interaction is inferred when mutations in separate genes, each of which alone causes a minimal phenotype, result in a significant growth defect under a given condition when combined in the same cell.

Publication

Asymmetry of the budding yeast Tem1 GTPase at spindle poles is required for spindle positioning but not for mitotic exit.

Scarfone I, Venturetti M, Hotz M, Lengefeld J, Barral Y, Piatti S

The asymmetrically dividing yeast S. cerevisiae assembles a bipolar spindle well after establishing the future site of cell division (i.e., the bud neck) and the division axis (i.e., the mother-bud axis). A surveillance mechanism called spindle position checkpoint (SPOC) delays mitotic exit and cytokinesis until the spindle is properly positioned relative to the mother-bud axis, thereby ensuring the correct ploidy ... [more]

PLoS Genet. Feb. 01, 2015; 11(2);e1004938 [Pubmed: 25658911]

Throughput

Low Throughput

Ontology Terms

phenotype: vegetative growth (APO:0000106)

Additional Notes

SGA screen with TEM1-Q79L
Table 1

Related interactions

Interaction	Experimental Evidence Code	Dataset	Throughput	Score	Curated By	Notes
PAC10 TEM1	Negative Genetic Negative Genetic Mutations/deletions in separate genes, each of which alone causes a minimal phenotype, but when combined in the same cell results in a more severe fitness defect or lethality under a given condition. This term is reserved for high or low throughput studies with scores.	Costanzo M (2016)	High	-0.205	BioGRID	2044796

Curated By

BioGRID

Interaction Summary

TEM1

Gene Ontology Biological Process

Gene Ontology Molecular Function

GTPase activity [IDA]protein binding [IPI]

Gene Ontology Cellular Component

PAC10