BAIT

RPP4

DL4460C, FCAALL.47, recognition of peronospora parasitica 4, AT4G16860
protein RECOGNITION OF PERONOSPORA PARASITICA 4
GO Process (2)
GO Function (1)
GO Component (0)

Gene Ontology Molecular Function

Arabidopsis thaliana (Columbia)
PREY

HSP81-3

AtHsp90-3, AtHsp90.3, HEAT SHOCK PROTEIN 81.3, HEAT SHOCK PROTEIN 90-3, HEAT SHOCK PROTEIN 90.3, HSP81.2, Hsp81.3, MDA7.5, MDA7_5, heat shock protein 81-3, AT5G56010
Heat shock protein 90-3
GO Process (4)
GO Function (2)
GO Component (6)
Arabidopsis thaliana (Columbia)

FRET

An interaction is inferred when close proximity of interaction partners is detected by fluorescence resonance energy transfer between pairs of fluorophore-labeled molecules, such as occurs between CFP (donor) and YFP (acceptor) fusion proteins.

Publication

Arabidopsis HSP90 protein modulates RPP4-mediated temperature-dependent cell death and defense responses.

Bao F, Huang X, Zhu C, Zhang X, Li X, Yang S

Plant defense responses are regulated by temperature. In Arabidopsis, the chilling-sensitive mutant chs2-1 (rpp4-1d) contains a gain-of-function mutation in the TIR-NB-LRR (Toll and interleukin 1 receptor-nucleotide binding-leucine-rich repeat) gene, RPP4 (RECOGNITION OF PERONOSPORA PARASITICA 4), which leads to constitutive activation of the defense response at low temperatures. Here, we identified and characterized two suppressors of rpp4-1d from a genetic screen, ... [more]

New Phytol. Jun. 01, 2014; 202(4);1320-34 [Pubmed: 24611624]

Throughput

  • Low Throughput

Related interactions

InteractionExperimental Evidence CodeDatasetThroughputScoreCurated ByNotes
RPP4 HSP81-3
Affinity Capture-Western
Affinity Capture-Western

An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins.

Low-BioGRID
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Curated By

  • BioGRID