BAIT

CDPK1

ATCDPK1, CALCIUM-DEPENDENT PROTEIN KINASE, CPK10, F6A14.1, F6A14_1, calcium-dependent protein kinase 1, AT1G18890
calcium-dependent protein kinase 1
GO Process (3)
GO Function (4)
GO Component (1)

Gene Ontology Biological Process

Gene Ontology Molecular Function

Gene Ontology Cellular Component

Arabidopsis thaliana (Columbia)
PREY

CAM1

ACAM-1, K22F20.20, K22F20_20, TCH1, TOUCH 1, calmodulin 1, AT5G37780
calmodulin 1
GO Process (2)
GO Function (1)
GO Component (2)

Gene Ontology Biological Process

Gene Ontology Molecular Function

Gene Ontology Cellular Component

Arabidopsis thaliana (Columbia)

PCA

A Protein-Fragment Complementation Assay (PCA) is a protein-protein interaction assay in which a bait protein is expressed as fusion to one of the either N- or C- terminal peptide fragments of a reporter protein and prey protein is expressed as fusion to the complementary N- or C- terminal fragment of the same reporter protein. Interaction of bait and prey proteins bring together complementary fragments, which can then fold into an active reporter, e.g. the split-ubiquitin assay.

Publication

An optimized optogenetic clustering tool for probing protein interaction and function.

Taslimi A, Vrana JD, Chen D, Borinskaya S, Mayer BJ, Kennedy MJ, Tucker CL

The Arabidopsis photoreceptor cryptochrome 2 (CRY2) was previously used as an optogenetic module, allowing spatiotemporal control of cellular processes with light. Here we report the development of a new CRY2-derived optogenetic module, 'CRY2olig', which induces rapid, robust, and reversible protein oligomerization in response to light. Using this module, we developed a novel protein interaction assay, Light-Induced Co-clustering, that can be ... [more]

Nat Commun Sep. 19, 2014; 5(0);4925 [Pubmed: 25233328]

Throughput

  • Low Throughput

Curated By

  • BioGRID