UHRF1
Gene Ontology Biological Process
- cell proliferation [TAS]
- histone monoubiquitination [IDA]
- histone ubiquitination [ISO]
- maintenance of DNA methylation [IMP, ISO]
- negative regulation of transcription from RNA polymerase II promoter [IDA, ISO]
- positive regulation of cellular protein metabolic process [ISO]
- protein autoubiquitination [ISO]
- protein ubiquitination involved in ubiquitin-dependent protein catabolic process [ISO]
Gene Ontology Molecular Function- core promoter proximal region sequence-specific DNA binding [ISO]
- hemi-methylated DNA-binding [IDA, ISO]
- histone binding [ISO]
- identical protein binding [IPI]
- methyl-CpG binding [ISO]
- methylated histone binding [IDA, ISO]
- nucleosomal histone binding [IDA]
- protein binding [IPI]
- ubiquitin protein ligase activity [IDA]
- ubiquitin-protein transferase activity [IDA, ISO]
- zinc ion binding [ISO]
- core promoter proximal region sequence-specific DNA binding [ISO]
- hemi-methylated DNA-binding [IDA, ISO]
- histone binding [ISO]
- identical protein binding [IPI]
- methyl-CpG binding [ISO]
- methylated histone binding [IDA, ISO]
- nucleosomal histone binding [IDA]
- protein binding [IPI]
- ubiquitin protein ligase activity [IDA]
- ubiquitin-protein transferase activity [IDA, ISO]
- zinc ion binding [ISO]
Gene Ontology Cellular Component
TOP2A
Gene Ontology Biological Process
- ATP catabolic process [IGI, ISO]
- DNA ligation [ISO]
- DNA topological change [IDA, ISO]
- DNA unwinding involved in DNA replication [IBA]
- apoptotic chromosome condensation [IBA, ISO]
- cellular response to DNA damage stimulus [ISO]
- chromosome condensation [IMP]
- chromosome segregation [IDA, IMP, ISO]
- embryonic cleavage [IMP]
- hematopoietic progenitor cell differentiation [IMP]
- mitotic DNA integrity checkpoint [IBA]
- mitotic recombination [IBA]
- positive regulation of apoptotic process [ISO]
- positive regulation of single stranded viral RNA replication via double stranded DNA intermediate [ISO]
- positive regulation of transcription from RNA polymerase II promoter [IMP]
- positive regulation of viral genome replication [ISO]
- resolution of meiotic recombination intermediates [IBA]
- sister chromatid segregation [IBA]
Gene Ontology Molecular Function- ATP binding [ISO]
- DNA binding [ISO]
- DNA binding, bending [ISO]
- DNA topoisomerase activity [ISO]
- DNA topoisomerase type II (ATP-hydrolyzing) activity [IDA, IGI, ISO]
- DNA-dependent ATPase activity [ISO]
- chromatin binding [ISO]
- drug binding [ISO]
- enzyme binding [ISO]
- histone deacetylase binding [ISO]
- magnesium ion binding [ISO]
- poly(A) RNA binding [ISO]
- protein C-terminus binding [ISO]
- protein heterodimerization activity [ISO]
- protein homodimerization activity [ISO]
- protein kinase C binding [ISO]
- sequence-specific DNA binding [ISO]
- structure-specific DNA binding [ISO]
- ubiquitin binding [ISO]
- ATP binding [ISO]
- DNA binding [ISO]
- DNA binding, bending [ISO]
- DNA topoisomerase activity [ISO]
- DNA topoisomerase type II (ATP-hydrolyzing) activity [IDA, IGI, ISO]
- DNA-dependent ATPase activity [ISO]
- chromatin binding [ISO]
- drug binding [ISO]
- enzyme binding [ISO]
- histone deacetylase binding [ISO]
- magnesium ion binding [ISO]
- poly(A) RNA binding [ISO]
- protein C-terminus binding [ISO]
- protein heterodimerization activity [ISO]
- protein homodimerization activity [ISO]
- protein kinase C binding [ISO]
- sequence-specific DNA binding [ISO]
- structure-specific DNA binding [ISO]
- ubiquitin binding [ISO]
Gene Ontology Cellular Component
Affinity Capture-Western
An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins.
Publication
Topoisomerase II regulates the maintenance of DNA methylation.
The maintenance of DNA methylation in nascent DNA is a critical event for numerous biological processes. Following DNA replication, DNMT1 is the key enzyme that strictly copies the methylation pattern from the parental strand to the nascent DNA. However, the mechanism underlying this highly specific event is not thoroughly understood. In this study, we identified topoisomerase IIα (TopoIIα) as a ... [more]
Throughput
- Low Throughput
Related interactions
| Interaction | Experimental Evidence Code | Dataset | Throughput | Score | Curated By | Notes |
|---|---|---|---|---|---|---|
| UHRF1 TOP2A | Affinity Capture-MS Affinity Capture-MS An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods. | High | - | BioGRID | - | |
| UHRF1 TOP2A | Co-localization Co-localization Interaction inferred from two proteins that co-localize in the cell by indirect immunofluorescence only when in addition, if one gene is deleted, the other protein becomes mis-localized. Also includes co-dependent association of proteins with promoter DNA in chromatin immunoprecipitation experiments. | Low | - | BioGRID | - |
Curated By
- BioGRID