BAIT

COQ3

hexaprenyldihydroxybenzoate methyltransferase, L000000380, YOL096C
O-methyltransferase; catalyzes two different O-methylation steps in ubiquinone (Coenzyme Q) biosynthesis; component of a mitochondrial ubiquinone-synthesizing complex; phosphoprotein
GO Process (1)
GO Function (2)
GO Component (3)
Saccharomyces cerevisiae (S288c)
PREY

SSA3

YG106, Hsp70 family ATPase SSA3, L000002071, L000000820, YBL075C
ATPase involved in protein folding and the response to stress; plays a role in SRP-dependent cotranslational protein-membrane targeting and translocation; member of the heat shock protein 70 (HSP70) family; localized to the cytoplasm; SSA3 has a paralog, SSA4, that arose from the whole genome duplication
GO Process (3)
GO Function (2)
GO Component (1)
Saccharomyces cerevisiae (S288c)

Affinity Capture-MS

An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.

Publication

Identification of Coq11, a new coenzyme Q biosynthetic protein in the CoQ-synthome in Saccharomyces cerevisiae.

Allan CM, Awad AM, Johnson JS, Shirasaki DI, Wang C, Blaby-Haas CE, Merchant SS, Loo JA, Clarke CF

Coenzyme Q (Q or ubiquinone) is a redox active lipid composed of a fully substituted benzoquinone ring and a polyisoprenoid tail and is required for mitochondrial electron transport. In the yeast Saccharomyces cerevisiae, Q is synthesized by the products of 11 known genes, COQ1-COQ9, YAH1, and ARH1. The function of some of the Coq proteins remains unknown, and several steps ... [more]

J. Biol. Chem. Mar. 20, 2015; 290(12);7517-34 [Pubmed: 25631044]

Throughput

  • High Throughput

Additional Notes

  • Table 3

Curated By

  • BioGRID