BAIT

AMBRA1

DCAF3, WDR94

autophagy/beclin-1 regulator 1

Autophagy Project

GO Process (4)

GO Function (2)

GO Component (5)

Gene Ontology Biological Process

autophagy [IBA]

positive regulation of mitochondrion degradation [IDA, IGI, IMP]

positive regulation of phosphatidylinositol 3-kinase activity [IDA]

regulation of autophagic vacuole assembly [IMP]

Gene Ontology Molecular Function

protein binding [IPI]
ubiquitin protein ligase binding [IPI]

Gene Ontology Cellular Component

cytoplasm [IDA]

intracellular membrane-bounded organelle [IBA]

mitochondrion [IDA, IMP]

CRISPR Database HGNC Alliance of Genome Resources OMIM Entrez Gene RefSeq UniprotKB Ensembl

Homo sapiens

PREY

PSMD12

Rpn5, p55

proteasome (prosome, macropain) 26S subunit, non-ATPase, 12

GO Process (21)

GO Function (0)

GO Component (6)

Gene Ontology Biological Process

DNA damage response, signal transduction by p53 class mediator resulting in cell cycle arrest [TAS]

G1/S transition of mitotic cell cycle [TAS]

RNA metabolic process [TAS]

anaphase-promoting complex-dependent proteasomal ubiquitin-dependent protein catabolic process [TAS]

antigen processing and presentation of exogenous peptide antigen via MHC class I [TAS]

antigen processing and presentation of exogenous peptide antigen via MHC class I, TAP-dependent [TAS]

antigen processing and presentation of peptide antigen via MHC class I [TAS]

apoptotic process [TAS]

cellular nitrogen compound metabolic process [TAS]

gene expression [TAS]

mRNA metabolic process [TAS]

mitotic cell cycle [TAS]

negative regulation of apoptotic process [TAS]

negative regulation of ubiquitin-protein ligase activity involved in mitotic cell cycle [TAS]

positive regulation of ubiquitin-protein ligase activity involved in mitotic cell cycle [TAS]

protein polyubiquitination [TAS]

regulation of apoptotic process [TAS]

regulation of cellular amino acid metabolic process [TAS]

regulation of ubiquitin-protein ligase activity involved in mitotic cell cycle [TAS]

small molecule metabolic process [TAS]

viral process [TAS]

Gene Ontology Cellular Component

extracellular vesicular exosome [IDA]

nucleoplasm [TAS]

proteasome accessory complex [ISS]

proteasome complex [IDA]

CRISPR Database HGNC Alliance of Genome Resources OMIM Entrez Gene RefSeq UniprotKB

Homo sapiens

Affinity Capture-MS

An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.

Publication

AMBRA1 interplay with cullin E3 ubiquitin ligases regulates autophagy dynamics.

Antonioli M, Albiero F, Nazio F, Vescovo T, Perdomo AB, Corazzari M, Marsella C, Piselli P, Gretzmeier C, Dengjel J, Cecconi F, Piacentini M, Fimia GM

Autophagy maintains cellular homeostasis by degrading harmful or unnecessary intracellular components. How the autophagy response is induced rapidly and transiently remains largely unknown. We report that the E3 ubiquitin ligases Cullin-5 and Cullin-4 regulate the onset and termination of autophagy, respectively, by dynamically interacting with AMBRA1, a regulator of autophagy. Under normal conditions, Cullin-4 binding to AMBRA1 limits its protein ... [more]

Dev. Cell Dec. 22, 2014; 31(6);734-46 [Pubmed: 25499913]

Throughput

High Throughput

Related interactions

Interaction	Experimental Evidence Code	Dataset	Throughput	Score	Curated By	Notes
AMBRA1 PSMD12	Proximity Label-MS Proximity Label-MS An interaction is inferred when a bait-enzyme fusion protein selectively modifies a vicinal protein with a diffusible reactive product, followed by affinity capture of the modified protein and identification by mass spectrometric methods.	Yamanaka S (2026)	High	-	BioGRID	3896760

Curated By

BioGRID