An interaction is inferred from the biochemical effect of one protein upon another, for example, GTP-GDP exchange activity or phosphorylation of a substrate by a kinase. The bait protein executes the activity on the substrate hit protein. A Modification value is recorded for interactions of this type with the possible values Phosphorylation, Ubiquitination, Sumoylation, Dephosphorylation, Methylation, Prenylation, Acetylation, Deubiquitination, Proteolytic Processing, Glucosylation, Nedd(Rub1)ylation, Deacetylation, No Modification, Demethylation.

Publication

AMBRA1 interplay with cullin E3 ubiquitin ligases regulates autophagy dynamics.

Antonioli M, Albiero F, Nazio F, Vescovo T, Perdomo AB, Corazzari M, Marsella C, Piselli P, Gretzmeier C, Dengjel J, Cecconi F, Piacentini M, Fimia GM

Autophagy maintains cellular homeostasis by degrading harmful or unnecessary intracellular components. How the autophagy response is induced rapidly and transiently remains largely unknown. We report that the E3 ubiquitin ligases Cullin-5 and Cullin-4 regulate the onset and termination of autophagy, respectively, by dynamically interacting with AMBRA1, a regulator of autophagy. Under normal conditions, Cullin-4 binding to AMBRA1 limits its protein ... [more]

Dev. Cell Dec. 22, 2014; 31(6);734-46 [Pubmed: 25499913]

Throughput

Low Throughput

Curated By

BioGRID

Interaction Summary

CUL5

Gene Ontology Biological Process

Gene Ontology Molecular Function

calcium channel activity [TAS]protein binding [IPI]receptor activity [TAS]ubiquitin protein ligase binding [IDA]

Gene Ontology Cellular Component

DEPTOR