EDS1
Gene Ontology Biological Process
- aerenchyma formation [IMP]
- defense response [ISS]
- lipid metabolic process [ISS]
- plant-type hypersensitive response [IMP]
- positive regulation of cell death [IMP]
- regulation of hydrogen peroxide metabolic process [IMP]
- response to hypoxia [IMP]
- response to singlet oxygen [IGI]
- systemic acquired resistance [IEP]
- systemic acquired resistance, salicylic acid mediated signaling pathway [IGI]
Gene Ontology Molecular Function
PAD4
Gene Ontology Biological Process
- aerenchyma formation [IMP]
- defense response to bacterium, incompatible interaction [IMP]
- defense response to insect [IGI, IMP]
- leaf senescence [IMP]
- lipid metabolic process [ISS]
- negative regulation of defense response [IMP]
- positive regulation of cell death [IMP]
- regulation of hydrogen peroxide metabolic process [IMP]
- response to hypoxia [IMP]
- response to insect [IMP]
- response to other organism [IEP]
- systemic acquired resistance [IEP]
- systemic acquired resistance, salicylic acid mediated signaling pathway [IMP, TAS]
Gene Ontology Molecular Function
FRET
An interaction is inferred when close proximity of interaction partners is detected by fluorescence resonance energy transfer between pairs of fluorophore-labeled molecules, such as occurs between CFP (donor) and YFP (acceptor) fusion proteins.
Publication
Functions of EDS1-like and PAD4 genes in grapevine defenses against powdery mildew.
The molecular interactions between grapevine and the obligate biotrophic fungus Erysiphe necator are not understood in depth. One reason for this is the recalcitrance of grapevine to genetic modifications. Using defense-related Arabidopsis mutants that are susceptible to pathogens, we were able to analyze key components in grapevine defense responses. We have examined the functions of defense genes associated with the ... [more]
Throughput
- Low Throughput
Related interactions
Interaction | Experimental Evidence Code | Dataset | Throughput | Score | Curated By | Notes |
---|---|---|---|---|---|---|
EDS1 PAD4 | Affinity Capture-Western Affinity Capture-Western An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins. | Low | - | BioGRID | - | |
EDS1 PAD4 | Co-fractionation Co-fractionation Interaction inferred from the presence of two or more protein subunits in a partially purified protein preparation. If co-fractionation is demonstrated between 3 or more proteins, then add them as a complex. | Low | - | BioGRID | - | |
PAD4 EDS1 | Co-purification Co-purification An interaction is inferred from the identification of two or more protein subunits in a purified protein complex, as obtained by classical biochemical fractionation or affinity purification and one or more additional fractionation steps. | Low | - | BioGRID | - | |
EDS1 PAD4 | FRET FRET An interaction is inferred when close proximity of interaction partners is detected by fluorescence resonance energy transfer between pairs of fluorophore-labeled molecules, such as occurs between CFP (donor) and YFP (acceptor) fusion proteins. | Low | - | BioGRID | - | |
PAD4 EDS1 | Reconstituted Complex Reconstituted Complex An interaction is detected between purified proteins in vitro. | Low | - | BioGRID | - | |
PAD4 EDS1 | Two-hybrid Two-hybrid Bait protein expressed as a DNA binding domain (DBD) fusion and prey expressed as a transcriptional activation domain (TAD) fusion and interaction measured by reporter gene activation. | Low | - | BioGRID | - | |
PAD4 EDS1 | Two-hybrid Two-hybrid Bait protein expressed as a DNA binding domain (DBD) fusion and prey expressed as a transcriptional activation domain (TAD) fusion and interaction measured by reporter gene activation. | Low | - | BioGRID | - |
Curated By
- BioGRID