Bait protein expressed as a DNA binding domain (DBD) fusion and prey expressed as a transcriptional activation domain (TAD) fusion and interaction measured by reporter gene activation.

Publication

Mechanistic insights into PTS2-mediated peroxisomal protein import: the co-receptor PEX5L drastically increases the interaction strength between the cargo protein and the receptor PEX7.

Kunze M, Malkani N, Maurer-Stroh S, Wiesinger C, Schmid JA, Berger J

The destination of peroxisomal matrix proteins is encoded by short peptide sequences, which have been characterized as peroxisomal targeting signals (PTS) residing either at the C terminus (PTS1) or close to the N terminus (PTS2). PTS2-carrying proteins interact with their cognate receptor protein PEX7 that mediates their transport to peroxisomes by a concerted action with a co-receptor protein, which in ... [more]

J. Biol. Chem. Feb. 20, 2015; 290(8);4928-40 [Pubmed: 25538232]

Throughput

Low Throughput

Curated By

BioGRID

Interaction Summary

PEX7

Gene Ontology Biological Process

Gene Ontology Molecular Function

enzyme binding [IPI]peroxisome matrix targeting signal-2 binding [IDA]protein homodimerization activity [IDA]

Gene Ontology Cellular Component

PEX5L

Gene Ontology Biological Process

Gene Ontology Molecular Function

peroxisome matrix targeting signal-1 binding [IBA, IDA]peroxisome targeting sequence binding [IDA]small GTPase binding [IPI]

Gene Ontology Cellular Component

Two-hybrid

Publication

Mechanistic insights into PTS2-mediated peroxisomal protein import: the co-receptor PEX5L drastically increases the interaction strength between the cargo protein and the receptor PEX7.

Throughput

Curated By

enzyme binding [IPI]
peroxisome matrix targeting signal-2 binding [IDA]
protein homodimerization activity [IDA]

peroxisome matrix targeting signal-1 binding [IBA, IDA]
peroxisome targeting sequence binding [IDA]
small GTPase binding [IPI]