VPRBP
Gene Ontology Biological Process
Gene Ontology Molecular Function
TET1
Gene Ontology Biological Process
- DNA demethylation [IDA, IMP, ISO]
- inner cell mass cell differentiation [IMP]
- negative regulation of methylation-dependent chromatin silencing [ISO]
- oxidation-reduction process [IDA]
- positive regulation of transcription from RNA polymerase II promoter [IMP]
- protein O-linked glycosylation [IMP]
- regulation of DNA methylation [IMP]
- regulation of genetic imprinting [IMP]
- stem cell maintenance [IMP]
Gene Ontology Molecular Function
Affinity Capture-Western
An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins.
Publication
CRL4(VprBP) E3 ligase promotes monoubiquitylation and chromatin binding of TET dioxygenases.
DNA methylation at the C-5 position of cytosine (5mC) regulates gene expression and plays pivotal roles in various biological processes. The TET dioxygenases catalyze iterative oxidation of 5mC, leading to eventual demethylation. Inactivation of TET enzymes causes multistage developmental defects, impaired cell reprogramming, and hematopoietic malignancies. However, little is known about how TET activity is regulated. Here we show that all three ... [more]
Throughput
- Low Throughput
Curated By
- BioGRID