BAIT
FAU1
5-formyltetrahydrofolate cyclo-ligase, YER183C
5,10-methenyltetrahydrofolate synthetase; involved in folic acid biosynthesis
GO Process (1)
GO Function (1)
GO Component (1)
Gene Ontology Biological Process
Gene Ontology Molecular Function
Gene Ontology Cellular Component
Saccharomyces cerevisiae (S288c)
PREY
MET6
5-methyltetrahydropteroyltriglutamate-homocysteine S-methyltransferase, L000001081, YER091C
Cobalamin-independent methionine synthase; involved in methionine biosynthesis and regeneration; requires a minimum of two glutamates on the methyltetrahydrofolate substrate, similar to bacterial metE homologs
GO Process (1)
GO Function (1)
GO Component (2)
Gene Ontology Biological Process
Gene Ontology Molecular Function
Gene Ontology Cellular Component
Saccharomyces cerevisiae (S288c)
Dosage Rescue
A genetic interaction is inferred when over expression or increased dosage of one gene rescues the lethality or growth defect of a strain that is mutated or deleted for another gene.
Publication
Serine hydroxymethyltransferase: a key player connecting purine, folate and methionine metabolism in Saccharomyces cerevisiae.
Previous genetic analyses showed phenotypic interactions between 5-amino-4-imidazole carboxamide ribonucleotide 5'-phosphate (AICAR) produced from the purine and histidine pathways and methionine biosynthesis. Here, we revisited the effect of AICAR on methionine requirement due to AICAR accumulation in the presence of the fau1 mutation invalidating folinic acid remobilization. We found that this methionine auxotrophy could be suppressed by overexpression of the ... [more]
Curr. Genet. Apr. 17, 2015; 0(0); [Pubmed: 25893566]
Throughput
- Low Throughput
Ontology Terms
- phenotype: auxotrophy (APO:0000097)
- phenotype: vegetative growth (APO:0000106)
Additional Notes
- Figure 3
- MET6 overexpression suppresses methionine auxotrophy of ade3 fau1 double mutant
- genetic complex
Curated By
- BioGRID