GCLM
Gene Ontology Biological Process
- cellular nitrogen compound metabolic process [TAS]
- glutamate metabolic process [IDA]
- glutathione biosynthetic process [IDA, IMP, TAS]
- glutathione derivative biosynthetic process [TAS]
- positive regulation of glutamate-cysteine ligase activity [IBA]
- regulation of blood vessel size [IMP]
- response to drug [IDA]
- response to oxidative stress [IDA]
- small molecule metabolic process [TAS]
- sulfur amino acid metabolic process [TAS]
- xenobiotic metabolic process [TAS]
Gene Ontology Molecular Function
Gene Ontology Cellular Component
GCLC
Gene Ontology Biological Process
- cell redox homeostasis [IDA]
- cellular nitrogen compound metabolic process [TAS]
- cysteine metabolic process [IDA]
- glutamate metabolic process [IDA]
- glutathione biosynthetic process [IDA, IMP, TAS]
- glutathione derivative biosynthetic process [TAS]
- negative regulation of apoptotic process [IDA]
- negative regulation of transcription, DNA-templated [IDA]
- regulation of blood vessel size [IMP]
- response to heat [IDA]
- response to hormone [IDA]
- response to oxidative stress [IDA]
- small molecule metabolic process [TAS]
- sulfur amino acid metabolic process [TAS]
- xenobiotic metabolic process [TAS]
Gene Ontology Molecular Function
Gene Ontology Cellular Component
Co-fractionation
Interaction inferred from the presence of two or more protein subunits in a partially purified protein preparation. If co-fractionation is demonstrated between 3 or more proteins, then add them as a complex.
Publication
Panorama of ancient metazoan macromolecular complexes
Macromolecular complexes are essential to conserved biological processes, but their prevalence across animals is unclear. By combining extensive biochemical fractionation with quantitative mass spectrometry, here we directly examined the composition of soluble multiprotein complexes among diverse metazoan models. Using an integrative approach, we generated a draft conservation map consisting of more than one million putative high-confidence co-complex interactions for species ... [more]
Quantitative Score
- 0.999999997 [Confidence Score]
Throughput
- High Throughput
Additional Notes
- Fractionation was combined with mass spectrometry from five diverse animal species to predict co-complex protein interactions conserved across metazoa using an integrative computational scoring procedure along with an SVM approach. The significant data set of 16655 PPI, was derived from a set of more than 1M interactions by examining a ROC curve of predicted interactions against reference annotated complexes at a 67.5% cumulative precision.
Related interactions
| Interaction | Experimental Evidence Code | Dataset | Throughput | Score | Curated By | Notes |
|---|---|---|---|---|---|---|
| GCLC GCLM | Affinity Capture-MS Affinity Capture-MS An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods. | High | 1 | BioGRID | 2218607 | |
| GCLM GCLC | Affinity Capture-MS Affinity Capture-MS An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods. | High | 1 | BioGRID | 3188796 | |
| GCLC GCLM | Affinity Capture-MS Affinity Capture-MS An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods. | High | 1 | BioGRID | 3038331 | |
| GCLC GCLM | Co-purification Co-purification An interaction is inferred from the identification of two or more protein subunits in a purified protein complex, as obtained by classical biochemical fractionation or affinity purification and one or more additional fractionation steps. | Low | - | BioGRID | 858424 | |
| GCLC GCLM | Reconstituted Complex Reconstituted Complex An interaction is inferred between proteins in vitro. This can include proteins in recombinant form or proteins isolated directly from cells with recombinant or purified bait. For example, GST pull-down assays where a GST-tagged protein is first isolated and then used to fish interactors from cell lysates are considered reconstituted complexes (e.g. PUBMED: 14657240, Fig. 4A or PUBMED: 14761940, Fig. 5). This can also include gel-shifts, surface plasmon resonance, isothermal titration calorimetry (ITC) and bio-layer interferometry (BLI) experiments. The bait-hit directionality may not be clear for 2 interacting proteins. In these cases the directionality is up to the discretion of the curator. | Low | - | BioGRID | - |
Curated By
- BioGRID