BAIT
SMEK1
FLFL1, KIAA2010, PP4R3A, smk-1, smk1, MSTP033
SMEK homolog 1, suppressor of mek1 (Dictyostelium)
GO Process (0)
GO Function (0)
GO Component (0)
Homo sapiens
PREY
SNX12
sorting nexin 12
GO Process (8)
GO Function (3)
GO Component (4)
Gene Ontology Biological Process
- endocytosis [IBA]
- negative regulation of early endosome to late endosome transport [IDA]
- negative regulation of gene expression [IMP]
- negative regulation of protein catabolic process [IDA]
- negative regulation of protein processing [IDA]
- negative regulation of protein transport [IDA]
- regulation of endocytosis [IMP]
- vesicle organization [IBA]
Gene Ontology Molecular Function
Gene Ontology Cellular Component
Homo sapiens
Co-fractionation
Interaction inferred from the presence of two or more protein subunits in a partially purified protein preparation. If co-fractionation is demonstrated between 3 or more proteins, then add them as a complex.
Publication
Panorama of ancient metazoan macromolecular complexes
Macromolecular complexes are essential to conserved biological processes, but their prevalence across animals is unclear. By combining extensive biochemical fractionation with quantitative mass spectrometry, here we directly examined the composition of soluble multiprotein complexes among diverse metazoan models. Using an integrative approach, we generated a draft conservation map consisting of more than one million putative high-confidence co-complex interactions for species ... [more]
Nature Sep. 17, 2015; 525(7569);339-44 [Pubmed: 26344197]
Quantitative Score
- 0.074501348 [Confidence Score]
Throughput
- High Throughput
Additional Notes
- Fractionation was combined with mass spectrometry from five diverse animal species to predict co-complex protein interactions conserved across metazoa using an integrative computational scoring procedure along with an SVM approach. The significant data set of 16655 PPI, was derived from a set of more than 1M interactions by examining a ROC curve of predicted interactions against reference annotated complexes at a 67.5% cumulative precision.
Curated By
- BioGRID