Protein-peptide

An interaction is detected between a protein and a peptide derived from an interaction partner. This includes phage display experiments.

Publication

Substrate specificities and identification of putative substrates of ATM kinase family members.

Kim ST, Lim DS, Canman CE, Kastan MB

Ataxia telangiectasia mutated (ATM) phosphorylates p53 protein in response to ionizing radiation, but the complex phenotype of AT cells suggests that it must have other cellular substrates as well. To identify substrates for ATM and the related kinases ATR and DNA-PK, we optimized in vitro kinase assays and developed a rapid peptide screening method to determine general phosphorylation consensus sequences. ... [more]

J. Biol. Chem. Dec. 31, 1999; 274(53);37538-43 [Pubmed: 10608806]

Throughput

  • Low Throughput

Additional Notes

  • in vitro kinase assay using GST-peptide substrates

Curated By

  • BioGRID