An interaction is inferred between proteins in vitro. This can include proteins in recombinant form or proteins isolated directly from cells with recombinant or purified bait. For example, GST pull-down assays where a GST-tagged protein is first isolated and then used to fish interactors from cell lysates are considered reconstituted complexes (e.g. PUBMED: 14657240, Fig. 4A or PUBMED: 14761940, Fig. 5). This can also include gel-shifts, surface plasmon resonance, isothermal titration calorimetry (ITC) and bio-layer interferometry (BLI) experiments. The bait-hit directionality may not be clear for 2 interacting proteins. In these cases the directionality is up to the discretion of the curator.

Publication

The histone-binding protein COPR5 is required for nuclear functions of the protein arginine methyltransferase PRMT5.

Lacroix M, El Messaoudi S, Rodier G, Le Cam A, Sardet C, Fabbrizio E

Protein arginine methyltransferase 5 (PRMT5) targets nuclear and cytoplasmic proteins. Here, we identified a nuclear protein, called cooperator of PRMT5 (COPR5), involved in the nuclear functions of PRMT5. COPR5 tightly binds to PRMT5, both in vitro and in living cells, but not to other members of the PRMT family. PRMT5 bound to COPR5 methylates histone H4 (R3) preferentially when compared ... [more]

EMBO Rep. May. 01, 2008; 9(5);452-8 [Pubmed: 18404153]

Throughput

Low Throughput

Curated By

BioGRID

Interaction Summary

COPRS

Gene Ontology Biological Process

Gene Ontology Molecular Function

histone binding [IDA]protein binding [IPI]

Gene Ontology Cellular Component

HIST3H3