RNF138
Gene Ontology Molecular Function
TCF7L2
Gene Ontology Biological Process
- blood vessel development [IMP]
- brain development [IBA]
- canonical Wnt signaling pathway [IC]
- canonical Wnt signaling pathway involved in positive regulation of epithelial to mesenchymal transition [IMP]
- cell cycle arrest [IMP]
- cell proliferation [IMP]
- fat cell differentiation [IDA]
- generation of neurons [IBA]
- glucose homeostasis [IDA]
- maintenance of DNA repeat elements [IMP]
- myoblast fate commitment [IDA]
- negative regulation of canonical Wnt signaling pathway [IMP]
- negative regulation of extrinsic apoptotic signaling pathway [IDA]
- negative regulation of sequence-specific DNA binding transcription factor activity [IDA]
- negative regulation of transcription from RNA polymerase II promoter [IDA]
- negative regulation of transcription, DNA-templated [IDA, IMP]
- negative regulation of type B pancreatic cell apoptotic process [IDA]
- pancreas development [TAS]
- positive regulation of heparan sulfate proteoglycan biosynthetic process [IMP]
- positive regulation of insulin secretion [IDA, IMP]
- positive regulation of protein binding [IDA]
- positive regulation of protein export from nucleus [IMP]
- positive regulation of protein kinase B signaling [IMP]
- positive regulation of transcription from RNA polymerase II promoter [IDA]
- regulation of hormone metabolic process [IDA]
- regulation of smooth muscle cell proliferation [IMP]
- regulation of transcription from RNA polymerase II promoter [IDA]
- response to glucose [ISS]
Gene Ontology Molecular Function- RNA polymerase II core promoter proximal region sequence-specific DNA binding [IDA]
- RNA polymerase II repressing transcription factor binding [IPI]
- armadillo repeat domain binding [IPI]
- beta-catenin binding [IDA, IPI]
- chromatin binding [IBA]
- gamma-catenin binding [IPI]
- nuclear hormone receptor binding [IPI]
- protein binding [IPI]
- protein kinase binding [IPI]
- sequence-specific DNA binding [IDA, IMP]
- sequence-specific DNA binding transcription factor activity [IDA]
- transcription factor binding [IPI]
- transcription regulatory region DNA binding [IDA]
- RNA polymerase II core promoter proximal region sequence-specific DNA binding [IDA]
- RNA polymerase II repressing transcription factor binding [IPI]
- armadillo repeat domain binding [IPI]
- beta-catenin binding [IDA, IPI]
- chromatin binding [IBA]
- gamma-catenin binding [IPI]
- nuclear hormone receptor binding [IPI]
- protein binding [IPI]
- protein kinase binding [IPI]
- sequence-specific DNA binding [IDA, IMP]
- sequence-specific DNA binding transcription factor activity [IDA]
- transcription factor binding [IPI]
- transcription regulatory region DNA binding [IDA]
Gene Ontology Cellular Component
Biochemical Activity (Ubiquitination)
An interaction is inferred from the biochemical effect of one protein upon another, for example, GTP-GDP exchange activity or phosphorylation of a substrate by a kinase. The bait protein executes the activity on the substrate hit protein. A Modification value is recorded for interactions of this type with the possible values Phosphorylation, Ubiquitination, Sumoylation, Dephosphorylation, Methylation, Prenylation, Acetylation, Deubiquitination, Proteolytic Processing, Glucosylation, Nedd(Rub1)ylation, Deacetylation, No Modification, Demethylation.
Publication
NARF, an nemo-like kinase (NLK)-associated ring finger protein regulates the ubiquitylation and degradation of T cell factor/lymphoid enhancer factor (TCF/LEF).
beta-Catenin is a key player in the Wnt signaling pathway, and interacts with cofactor T cell factor/lymphoid enhancer factor (TCF/LEF) to generate a transcription activator complex that activates Wnt-induced genes. We previously reported that Nemo-like kinase (NLK) negatively regulates Wnt signaling via phosphorylation of TCF/LEF. To further evaluate the physiological roles of NLK, we performed yeast two-hybrid screening to identify ... [more]
Throughput
- Low Throughput
Additional Notes
- E1: rabbit
- E2: E2-25K
- Figure 3
Related interactions
| Interaction | Experimental Evidence Code | Dataset | Throughput | Score | Curated By | Notes |
|---|---|---|---|---|---|---|
| TCF7L2 RNF138 | Affinity Capture-Western Affinity Capture-Western An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins. | Low | - | BioGRID | 1277838 |
Curated By
- BioGRID