An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins.

Publication

Overlapping signals for protein degradation and nuclear localization define a role for intrinsic RAG-2 nuclear uptake in dividing cells.

Ross AE, Vuica M, Desiderio S

Expression of the recombinase proteins RAG-1 and RAG-2 is discordant: while RAG-1 is relatively long lived, RAG-2 is degraded periodically at the G(1)-S transition. Destruction of RAG-2 is mediated by a conserved interval in the recombination-dispensable region. The need for RAG-2 to reaccumulate in the nucleus at each cell division suggested the existence of an intrinsic RAG-2 nuclear localization signal ... [more]

Mol. Cell. Biol. Aug. 01, 2003; 23(15);5308-19 [Pubmed: 12861017]

Throughput

Low Throughput

Curated By

BioGRID

Interaction Summary

RAG2

Gene Ontology Biological Process

Gene Ontology Molecular Function

IPO5

Gene Ontology Biological Process

Gene Ontology Molecular Function

GTPase inhibitor activity [TAS]poly(A) RNA binding [IDA]protein binding [IPI]protein transporter activity [ISS]

Gene Ontology Cellular Component

Affinity Capture-Western

Publication

Overlapping signals for protein degradation and nuclear localization define a role for intrinsic RAG-2 nuclear uptake in dividing cells.

Throughput

Curated By

GTPase inhibitor activity [TAS]
poly(A) RNA binding [IDA]
protein binding [IPI]
protein transporter activity [ISS]