NR3C2
Gene Ontology Biological Process
Gene Ontology Molecular Function
Gene Ontology Cellular Component
NCOA1
Gene Ontology Biological Process
- androgen receptor signaling pathway [NAS]
- cellular lipid metabolic process [TAS]
- cellular response to hormone stimulus [IBA]
- intracellular receptor signaling pathway [IBA]
- positive regulation of transcription from RNA polymerase II promoter [IDA, NAS]
- positive regulation of transcription from RNA polymerase II promoter by galactose [IDA]
- positive regulation of transcription, DNA-templated [IDA, NAS]
- small molecule metabolic process [TAS]
- transcription, DNA-templated [IDA]
Gene Ontology Molecular Function- RNA polymerase II transcription coactivator activity [NAS]
- androgen receptor binding [NAS]
- enzyme binding [IPI]
- ligand-dependent nuclear receptor binding [IPI]
- ligand-dependent nuclear receptor transcription coactivator activity [IDA]
- nuclear hormone receptor binding [IDA]
- protein N-terminus binding [IPI]
- protein binding [IPI]
- transcription coactivator activity [IDA, NAS]
- RNA polymerase II transcription coactivator activity [NAS]
- androgen receptor binding [NAS]
- enzyme binding [IPI]
- ligand-dependent nuclear receptor binding [IPI]
- ligand-dependent nuclear receptor transcription coactivator activity [IDA]
- nuclear hormone receptor binding [IDA]
- protein N-terminus binding [IPI]
- protein binding [IPI]
- transcription coactivator activity [IDA, NAS]
Gene Ontology Cellular Component
Two-hybrid
Bait protein expressed as a DNA binding domain (DBD) fusion and prey expressed as a transcriptional activation domain (TAD) fusion and interaction measured by reporter gene activation.
Publication
Automated yeast two-hybrid screening for nuclear receptor-interacting proteins.
High throughput analysis of protein-protein interactions is an important sector of hypothesis-generating research. Using an improved and automated version of the yeast two-hybrid system, we completed a large interaction screening project with a focus on nuclear receptors and their cofactors. A total of 425 independent yeast two-hybrid cDNA library screens resulted in 6425 potential interacting protein fragments involved in 1613 ... [more]
Throughput
- Low Throughput
Related interactions
| Interaction | Experimental Evidence Code | Dataset | Throughput | Score | Curated By | Notes |
|---|---|---|---|---|---|---|
| NR3C2 NCOA1 | Affinity Capture-Western Affinity Capture-Western An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins. | Low | - | BioGRID | - |
Curated By
- BioGRID