BAIT
NCOA1
F-SRC-1, KAT13A, RIP160, SRC1, bHLHe42, bHLHe74
nuclear receptor coactivator 1
GO Process (9)
GO Function (9)
GO Component (2)
Gene Ontology Biological Process
- androgen receptor signaling pathway [NAS]
- cellular lipid metabolic process [TAS]
- cellular response to hormone stimulus [IBA]
- intracellular receptor signaling pathway [IBA]
- positive regulation of transcription from RNA polymerase II promoter [IDA, NAS]
- positive regulation of transcription from RNA polymerase II promoter by galactose [IDA]
- positive regulation of transcription, DNA-templated [IDA, NAS]
- small molecule metabolic process [TAS]
- transcription, DNA-templated [IDA]
Gene Ontology Molecular Function- RNA polymerase II transcription coactivator activity [NAS]
- androgen receptor binding [NAS]
- enzyme binding [IPI]
- ligand-dependent nuclear receptor binding [IPI]
- ligand-dependent nuclear receptor transcription coactivator activity [IDA]
- nuclear hormone receptor binding [IDA]
- protein N-terminus binding [IPI]
- protein binding [IPI]
- transcription coactivator activity [IDA, NAS]
- RNA polymerase II transcription coactivator activity [NAS]
- androgen receptor binding [NAS]
- enzyme binding [IPI]
- ligand-dependent nuclear receptor binding [IPI]
- ligand-dependent nuclear receptor transcription coactivator activity [IDA]
- nuclear hormone receptor binding [IDA]
- protein N-terminus binding [IPI]
- protein binding [IPI]
- transcription coactivator activity [IDA, NAS]
Gene Ontology Cellular Component
Homo sapiens
PREY
VCP
ALS14, HEL-220, HEL-S-70, IBMPFD, IBMPFD1, TERA, p97
valosin containing protein
GO Process (18)
GO Function (8)
GO Component (13)
Gene Ontology Biological Process
- DNA repair [NAS]
- ER-associated ubiquitin-dependent protein catabolic process [IDA, IMP, TAS]
- activation of cysteine-type endopeptidase activity involved in apoptotic process [ISS]
- cellular response to DNA damage stimulus [IDA]
- double-strand break repair [IDA]
- endoplasmic reticulum unfolded protein response [TAS]
- establishment of protein localization [TAS]
- positive regulation of Lys63-specific deubiquitinase activity [IDA]
- positive regulation of proteasomal ubiquitin-dependent protein catabolic process [IDA]
- positive regulation of protein K63-linked deubiquitination [IDA]
- positive regulation of protein catabolic process [IDA]
- positive regulation of protein complex assembly [IDA]
- proteasome-mediated ubiquitin-dependent protein catabolic process [NAS]
- protein N-linked glycosylation via asparagine [IMP]
- protein ubiquitination [IDA, NAS]
- regulation of apoptotic process [TAS]
- retrograde protein transport, ER to cytosol [IDA]
- translesion synthesis [IMP]
Gene Ontology Molecular Function
Gene Ontology Cellular Component
- Hrd1p ubiquitin ligase complex [IDA]
- cytoplasm [IDA]
- cytosol [IDA]
- endoplasmic reticulum [IDA]
- endoplasmic reticulum membrane [IDA]
- extracellular vesicular exosome [IDA]
- intracellular membrane-bounded organelle [ISS]
- lipid particle [IDA]
- nucleoplasm [IDA]
- nucleus [IDA, TAS]
- perinuclear region of cytoplasm [IDA]
- proteasome complex [IDA]
- site of double-strand break [IDA]
Homo sapiens
Affinity Capture-MS
An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.
Publication
Proteomic analysis of steady-state nuclear hormone receptor coactivator complexes.
We report our initial efforts in the analysis of endogenous nuclear receptor coactivator complexes as a research bridging strand of the Nuclear Receptor Signaling Atlas (NURSA) (www.NURSA.org). A proteomic approach is used to systematically isolate a variety of coactivator complexes using HeLa cells as a model cell line and to identify the coactivator-associated proteins with mass spectrometry. We have isolated ... [more]
Mol. Endocrinol. Oct. 01, 2005; 19(10);2451-65 [Pubmed: 16051665]
Throughput
- Low Throughput
Ontology Terms
- cell line: hela cell (BTO:0000567) [cervical adenocarcinoma (DOID:3702)]
Curated By
- BioGRID