BAIT

PSMD7

MOV34, P40, Rpn8, S12
proteasome (prosome, macropain) 26S subunit, non-ATPase, 7
GO Process (21)
GO Function (2)
GO Component (6)
Homo sapiens
PREY

VPR

HIV1gp4
p15
GO Process (0)
GO Function (0)
GO Component (0)
Human Immunodeficiency Virus 1

Affinity Capture-Western

An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins.

Publication

HIV-1 Vpr interacts with a human 34-kDa mov34 homologue, a cellular factor linked to the G2/M phase transition of the mammalian cell cycle.

Mahalingam S, Ayyavoo V, Patel M, Kieber-Emmons T, Kao GD, Muschel RJ, Weiner DB

Several important and possibly interrelated functions have been identified for the HIV-1 accessory gene product Vpr. These include import of the HIV reverse transcription complex into the nucleus of nondividing cells, cellular differentiation including cell cycle arrest at the G2/M phase border, immune suppression, and enhancement of virus replication. We have cloned a candidate Vpr ligand, termed human Vpr interacting ... [more]

Proc. Natl. Acad. Sci. U.S.A. Mar. 31, 1998; 95(7);3419-24 [Pubmed: 9520381]

Throughput

  • Low Throughput

Curated By

  • BioGRID