Affinity Capture-Western

An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins.

Publication

SUMOylation regulates nuclear localization of Krueppel-like factor 5.

Du JX, Bialkowska AB, McConnell BB, Yang VW

SUMOylation is a form of post-translational modification shown to control nuclear transport. Krueppel-like factor 5 (KLF5) is an important mediator of cell proliferation and is primarily localized to the nucleus. Here we show that mouse KLF5 is SUMOylated at lysine residues 151 and 202. Mutation of these two lysines or two conserved nearby glutamates results in the loss of SUMOylation ... [more]

J. Biol. Chem. Nov. 14, 2008; 283(46);31991-2002 [Pubmed: 18782761]

Throughput

  • Low Throughput

Curated By

  • BioGRID