An interaction is inferred between proteins in vitro. This can include proteins in recombinant form or proteins isolated directly from cells with recombinant or purified bait. For example, GST pull-down assays where a GST-tagged protein is first isolated and then used to fish interactors from cell lysates are considered reconstituted complexes (e.g. PUBMED: 14657240, Fig. 4A or PUBMED: 14761940, Fig. 5). This can also include gel-shifts, surface plasmon resonance, isothermal titration calorimetry (ITC) and bio-layer interferometry (BLI) experiments. The bait-hit directionality may not be clear for 2 interacting proteins. In these cases the directionality is up to the discretion of the curator.

Publication

LGALS3BP, lectin galactoside-binding soluble 3 binding protein, promotes oncogenic cellular events impeded by antibody intervention.

Stampolidis P, Ullrich A, Iacobelli S

The extracellular matrix protein lectin galactoside-binding soluble 3 binding protein (LGALS3BP) constitutes a negative prognostic marker of cancer onset and progression with increasing value in clinical application. Since its discovery, however, although the glycoprotein has been implicated in a growing number of disease-related processes, its actual role and mechanism of action have remained ambiguous, thus hindering opportunities for therapeutic development. ... [more]

Oncogene Jan. 02, 2015; 34(1);39-52 [Pubmed: 24362527]

Throughput

Low Throughput

Curated By

BioGRID

Interaction Summary

ITGA5

Gene Ontology Biological Process

Gene Ontology Molecular Function

platelet-derived growth factor receptor binding [TAS]protein binding [IPI]vascular endothelial growth factor receptor 2 binding [TAS]

Gene Ontology Cellular Component

LGALS3BP