BAIT

BLM

BS, RECQ2, RECQL2, RECQL3
Bloom syndrome, RecQ helicase-like
Homo sapiens
PREY

AQR

IBP160, fSAP164
aquarius intron-binding spliceosomal factor
GO Process (1)
GO Function (2)
GO Component (4)

Gene Ontology Biological Process

Gene Ontology Molecular Function

Gene Ontology Cellular Component

Homo sapiens

Synthetic Growth Defect

A genetic interaction is inferred when mutations in separate genes, each of which alone causes a minimal phenotype, result in a significant growth defect under a given condition when combined in the same cell.

Publication

A negative genetic interaction map in isogenic cancer cell lines reveals cancer cell vulnerabilities.

Vizeacoumar FJ, Arnold R, Vizeacoumar FS, Chandrashekhar M, Buzina A, Young JT, Kwan JH, Sayad A, Mero P, Lawo S, Tanaka H, Brown KR, Baryshnikova A, Mak AB, Fedyshyn Y, Wang Y, Brito GC, Kasimer D, Makhnevych T, Ketela T, Datti A, Babu M, Emili A, Pelletier L, Wrana J, Wainberg Z, Kim PM, Rottapel R, O'Brien CA, Andrews B, Boone C, Moffat J

Improved efforts are necessary to define the functional product of cancer mutations currently being revealed through large-scale sequencing efforts. Using genome-scale pooled shRNA screening technology, we mapped negative genetic interactions across a set of isogenic cancer cell lines and confirmed hundreds of these interactions in orthogonal co-culture competition assays to generate a high-confidence genetic interaction network of differentially essential or ... [more]

Mol. Syst. Biol. Oct. 10, 2013; 9(0);696 [Pubmed: 24104479]

Throughput

  • High Throughput

Ontology Terms

  • phenotype: growth abnormality (HP:0001507) [hct-116 cell (BTO:0001109)]

Additional Notes

  • shRNA
  • siRNA

Curated By

  • BioGRID