A genetic interaction is inferred when mutations in separate genes, each of which alone causes a minimal phenotype, result in a significant growth defect under a given condition when combined in the same cell.

Publication

Whole genome RNAi screens reveal a critical role of REV3 in coping with replication stress.

Kotov IN, Siebring-van Olst E, Knobel PA, van der Meulen-Muileman IH, Felley-Bosco E, van Beusechem VW, Smit EF, Stahel RA, Marti TM

REV3, the catalytic subunit of translesion polymerase zeta (polÎ¶), is commonly associated with DNA damage bypass and repair. Despite sharing accessory subunits with replicative polymerase Î´, very little is known about the role of polÎ¶ in DNA replication. We previously demonstrated that inhibition of REV3 expression induces persistent DNA damage and growth arrest in cancer cells. To reveal determinants of ... [more]

Mol Oncol Dec. 01, 2014; 8(8);1747-59 [Pubmed: 25113059]

Quantitative Score

5.0581e-05 [Confidence Score]

Throughput

High Throughput

Ontology Terms

phenotype: growth abnormality (HP:0001507)

Additional Notes

RNAi screen for synthetic sickness/lethality in REV3-deficient cells
Table 1 and Table 2

Curated By

BioGRID

Interaction Summary

REV3L

Gene Ontology Biological Process

Gene Ontology Molecular Function

3'-5' exonuclease activity [IBA]DNA-directed DNA polymerase activity [IBA]protein binding [IPI]

Gene Ontology Cellular Component

ATAD1