BAIT

TORD

Y75_p0971
chaperone involved in maturation of TorA subunit of trimethylamine N-oxide reductase system I
GO Process (0)
GO Function (0)
GO Component (0)
Escherichia coli (K12/W3110)
PREY

YJHR

Y75_p4194
frameshift suppressor
GO Process (0)
GO Function (0)
GO Component (0)
Escherichia coli (K12/W3110)

Negative Genetic

Mutations/deletions in separate genes, each of which alone causes a minimal phenotype, but when combined in the same cell results in a more severe fitness defect or lethality under a given condition. This term is reserved for high or low throughput studies with scores.

Publication

Genetic interaction maps in Escherichia coli reveal functional crosstalk among cell envelope biogenesis pathways.

Babu M, Diaz-Mejia JJ, Vlasblom J, Gagarinova A, Phanse S, Graham C, Yousif F, Ding H, Xiong X, Nazarians-Armavil A, Alamgir M, Ali M, Pogoutse O, Pe'er A, Arnold R, Michaut M, Parkinson J, Golshani A, Whitfield C, Wodak SJ, Moreno-Hagelsieb G, Greenblatt JF, Emili A

As the interface between a microbe and its environment, the bacterial cell envelope has broad biological and clinical significance. While numerous biosynthesis genes and pathways have been identified and studied in isolation, how these intersect functionally to ensure envelope integrity during adaptive responses to environmental challenge remains unclear. To this end, we performed high-density synthetic genetic screens to generate quantitative ... [more]

PLoS Genet. Nov. 01, 2011; 7(11);e1002377 [Pubmed: 22125496]

Quantitative Score

  • -20.0000002 [Confidence Score]

Throughput

  • High Throughput

Additional Notes

  • Conditions: rich growth medium
  • Genetic interactions were considered significant if they had an E-score >= 2.0 for positive interactions (alleviating interactions) and E-score <= -2.0 for negative interactions (aggravating interactions).

Curated By

  • BioGRID