BAIT

TAF14

ANC1, SWP29, TAF30, TFG3, TATA-binding protein-associated factor TAF14, TafII30, L000000086, YPL129W
Subunit of TFIID, TFIIF, INO80, SWI/SNF, and NuA3 complexes; involved in RNA polymerase II transcription initiation and in chromatin modification; contains a YEATS domain
Saccharomyces cerevisiae (S288c)
PREY

NUT1

MED5, SSX4, L000003073, S000029517, L000002100, YGL151W
Component of the RNA polymerase II mediator complex; mediator is required for transcriptional activation and also has a role in basal transcription
GO Process (2)
GO Function (0)
GO Component (3)
Saccharomyces cerevisiae (S288c)

Affinity Capture-MS

An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.

Publication

Proteomics of the eukaryotic transcription machinery: identification of proteins associated with components of yeast TFIID by multidimensional mass spectrometry.

Sanders SL, Jennings J, Canutescu A, Link AJ, Weil PA

The general transcription factor TFIID is a multisubunit complex of TATA-binding protein (TBP) and 14 distinct TBP-associated factors (TAFs). Although TFIID constituents are required for transcription initiation of most mRNA encoding genes, the mechanism of TFIID action remains unclear. To gain insight into TFIID function, we sought to generate a proteomic catalogue of proteins specifically interacting with TFIID subunits. Toward ... [more]

Mol. Cell. Biol. Jul. 01, 2002; 22(13);4723-38 [Pubmed: 12052880]

Throughput

  • High Throughput

Related interactions

InteractionExperimental Evidence CodeDatasetThroughputScoreCurated ByNotes
TAF14 NUT1
Negative Genetic
Negative Genetic

Mutations/deletions in separate genes, each of which alone causes a minimal phenotype, but when combined in the same cell results in a more severe fitness defect or lethality under a given condition. This term is reserved for high or low throughput studies with scores.

High-3.2627BioGRID
221530
TAF14 NUT1
Negative Genetic
Negative Genetic

Mutations/deletions in separate genes, each of which alone causes a minimal phenotype, but when combined in the same cell results in a more severe fitness defect or lethality under a given condition. This term is reserved for high or low throughput studies with scores.

High-0.151BioGRID
2191488

Curated By

  • BioGRID