NHP6A
Gene Ontology Biological Process
- DNA replication-independent nucleosome organization [IDA]
- RNA polymerase II transcriptional preinitiation complex assembly [IDA]
- RNA polymerase III transcriptional preinitiation complex assembly [IDA, IGI]
- chromatin remodeling [IGI]
- maintenance of transcriptional fidelity during DNA-templated transcription elongation from RNA polymerase III promoter [IDA]
- mismatch repair [IC]
Gene Ontology Molecular Function
Gene Ontology Cellular Component
SPT16
Gene Ontology Biological Process
- DNA replication-independent nucleosome organization [IDA]
- DNA-dependent DNA replication [IPI]
- chromatin organization involved in regulation of transcription [IMP]
- nucleosome assembly [IDA]
- positive regulation of RNA polymerase II transcriptional preinitiation complex assembly [IDA]
- positive regulation of transcription initiation from RNA polymerase II promoter [IGI, IMP]
- transcription elongation from RNA polymerase II promoter [IPI]
Gene Ontology Molecular Function
Gene Ontology Cellular Component
Affinity Capture-Western
An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins.
Publication
A bipartite yeast SSRP1 analog comprised of Pob3 and Nhp6 proteins modulates transcription.
The FACT complex of vertebrate cells, comprising the Cdc68 (Spt16) and SSRP1 proteins, facilitates transcription elongation on a nucleosomal template and modulates the elongation-inhibitory effects of the DSIF complex in vitro. Genetic findings show that the related yeast (Saccharomyces cerevisiae) complex, termed CP, also mediates transcription. The CP components Cdc68 and Pob3 closely resemble the FACT components, except that the ... [more]
Throughput
- Low Throughput
Related interactions
| Interaction | Experimental Evidence Code | Dataset | Throughput | Score | Curated By | Notes |
|---|---|---|---|---|---|---|
| SPT16 NHP6A | Affinity Capture-Western Affinity Capture-Western An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins. | Low | - | BioGRID | - | |
| SPT16 NHP6A | Dosage Rescue Dosage Rescue A genetic interaction is inferred when over expression or increased dosage of one gene rescues the lethality or growth defect of a strain that is mutated or deleted for another gene. | Low | - | BioGRID | 262165 | |
| SPT16 NHP6A | Synthetic Growth Defect Synthetic Growth Defect A genetic interaction is inferred when mutations in separate genes, each of which alone causes a minimal phenotype, result in a significant growth defect under a given condition when combined in the same cell. | Low | - | BioGRID | 239591 | |
| NHP6A SPT16 | Synthetic Growth Defect Synthetic Growth Defect A genetic interaction is inferred when mutations in separate genes, each of which alone causes a minimal phenotype, result in a significant growth defect under a given condition when combined in the same cell. | Low | - | BioGRID | 157230 | |
| SPT16 NHP6A | Synthetic Growth Defect Synthetic Growth Defect A genetic interaction is inferred when mutations in separate genes, each of which alone causes a minimal phenotype, result in a significant growth defect under a given condition when combined in the same cell. | Low | - | BioGRID | 157400 | |
| SPT16 NHP6A | Synthetic Lethality Synthetic Lethality A genetic interaction is inferred when mutations or deletions in separate genes, each of which alone causes a minimal phenotype, result in lethality when combined in the same cell under a given condition. | Low | - | BioGRID | 164125 | |
| NHP6A SPT16 | Synthetic Lethality Synthetic Lethality A genetic interaction is inferred when mutations or deletions in separate genes, each of which alone causes a minimal phenotype, result in lethality when combined in the same cell under a given condition. | Low | - | BioGRID | 158356 |
Curated By
- BioGRID