BAIT

HHT1

BUR5, SIN2, histone H3, L000000772, YBR010W
Histone H3; core histone protein required for chromatin assembly, part of heterochromatin-mediated telomeric and HM silencing; one of two identical histone H3 proteins (see HHT2); regulated by acetylation, methylation, and phosphorylation; H3K14 acetylation plays an important role in the unfolding of strongly positioned nucleosomes during repair of UV damage
Saccharomyces cerevisiae (S288c)
PREY

ARP4

ACT3, L000000027, YJL081C
Nuclear actin-related protein involved in chromatin remodeling; component of chromatin-remodeling enzyme complexes
Saccharomyces cerevisiae (S288c)

Far Western

An interaction is detected between a protein immobilized on a membrane and a purified protein probe.

Publication

The nuclear actin-related protein of Saccharomyces cerevisiae, Act3p/Arp4, interacts with core histones.

Harata M, Oma Y, Mizuno S, Jiang YW, Stillman DJ, Wintersberger U

Act3p/Arp4, an essential actin-related protein of Saccharomyces cerevisiae located within the nucleus, is, according to genetic data, involved in transcriptional regulation. In addition to the basal core structure of the actin family members, which is responsible for ATPase activity, Act3p possesses two insertions, insertions I and II, the latter of which is predicted to form a loop-like structure protruding from ... [more]

Mol. Biol. Cell Aug. 01, 1999; 10(8);2595-605 [Pubmed: 10436015]

Throughput

  • Low Throughput

Related interactions

InteractionExperimental Evidence CodeDatasetThroughputScoreCurated ByNotes
ARP4 HHT1
Reconstituted Complex
Reconstituted Complex

An interaction is detected between purified proteins in vitro.

Low-BioGRID
890971
HHT1 ARP4
Reconstituted Complex
Reconstituted Complex

An interaction is detected between purified proteins in vitro.

Low-BioGRID
890982
ARP4 HHT1
Synthetic Lethality
Synthetic Lethality

A genetic interaction is inferred when mutations or deletions in separate genes, each of which alone causes a minimal phenotype, result in lethality when combined in the same cell under a given condition.

Low/High-BioGRID
283532
ARP4 HHT1
Two-hybrid
Two-hybrid

Bait protein expressed as a DNA binding domain (DBD) fusion and prey expressed as a transcriptional activation domain (TAD) fusion and interaction measured by reporter gene activation.

Low-BioGRID
-

Curated By

  • BioGRID