CYC8
Gene Ontology Biological Process
- chromatin remodeling [IGI]
- negative regulation of dipeptide transport by negative regulation of transcription from RNA polymerase II promoter [IMP]
- negative regulation of transcription from RNA polymerase II promoter [IMP]
- negative regulation of transcription from RNA polymerase II promoter during mitosis [IMP]
- nucleosome positioning [IDA]
- regulation of fatty acid biosynthetic process by regulation of transcription from RNA polymerase II promoter [IMP]
- regulation of response to DNA damage stimulus [IMP]
Gene Ontology Molecular Function- RNA polymerase II transcription factor binding transcription factor activity involved in negative regulation of transcription [IDA, IPI]
- RNA polymerase II transcription factor binding transcription factor activity involved in positive regulation of transcription [IDA, IGI, IPI]
- histone deacetylase binding [IDA]
- RNA polymerase II transcription factor binding transcription factor activity involved in negative regulation of transcription [IDA, IPI]
- RNA polymerase II transcription factor binding transcription factor activity involved in positive regulation of transcription [IDA, IGI, IPI]
- histone deacetylase binding [IDA]
Gene Ontology Cellular Component
AFT1
Gene Ontology Biological Process
- chromosome segregation [IMP]
- establishment of mitotic sister chromatid cohesion [IMP]
- meiotic chromosome segregation [IMP]
- positive regulation of iron ion transport [IMP]
- positive regulation of transcription from RNA polymerase II promoter [IDA]
- positive regulation of transcription from RNA polymerase II promoter in response to iron ion starvation [IMP]
Gene Ontology Molecular Function
Reconstituted Complex
An interaction is detected between purified proteins in vitro.
Publication
Nhp6 facilitates Aft1 binding and Ssn6 recruitment, both essential for FRE2 transcriptional activation.
We found Nhp6a/b yeast HMG-box chromatin-associated architectural factors and Ssn6 (Cyc8) corepressor to be crucial transcriptional coactivators of FRE2 gene. FRE2 encoding a plasma membrane ferric reductase is induced by the iron-responsive, DNA-binding, transcriptional activator Aft1. We have shown that Nhp6 interacts directly with the Aft1 N-half, including the DNA-binding region, to facilitate Aft1 binding at FRE2 UAS. Ssn6 also ... [more]
Throughput
- Low Throughput
Related interactions
| Interaction | Experimental Evidence Code | Dataset | Throughput | Score | Curated By | Notes |
|---|---|---|---|---|---|---|
| CYC8 AFT1 | Affinity Capture-Western Affinity Capture-Western An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins. | Low | - | BioGRID | - | |
| AFT1 CYC8 | Negative Genetic Negative Genetic Mutations/deletions in separate genes, each of which alone causes a minimal phenotype, but when combined in the same cell results in a more severe fitness defect or lethality under a given condition. This term is reserved for high or low throughput studies with scores. | High | -2.8444 | BioGRID | 509637 |
Curated By
- BioGRID