BAIT

YCBX

Y75_p0919
2Fe-2S cluster-containing protein
GO Process (0)
GO Function (0)
GO Component (0)
Escherichia coli (K12/W3110)
PREY

GLTA

Y75_p0700
citrate synthase
GO Process (0)
GO Function (0)
GO Component (0)
Escherichia coli (K12/W3110)

Negative Genetic

Mutations/deletions in separate genes, each of which alone causes a minimal phenotype, but when combined in the same cell results in a more severe fitness defect or lethality under a given condition. This term is reserved for high or low throughput studies with scores.

Publication

Quantitative genome-wide genetic interaction screens reveal global epistatic relationships of protein complexes in Escherichia coli.

Babu M, Arnold R, Bundalovic-Torma C, Gagarinova A, Wong KS, Kumar A, Stewart G, Samanfar B, Aoki H, Wagih O, Vlasblom J, Phanse S, Lad K, Yeou Hsiung Yu A, Graham C, Jin K, Brown E, Golshani A, Kim P, Moreno-Hagelsieb G, Greenblatt J, Houry WA, Parkinson J, Emili A

Large-scale proteomic analyses in Escherichia coli have documented the composition and physical relationships of multiprotein complexes, but not their functional organization into biological pathways and processes. Conversely, genetic interaction (GI) screens can provide insights into the biological role(s) of individual gene and higher order associations. Combining the information from both approaches should elucidate how complexes and pathways intersect functionally at ... [more]

PLoS Genet. Feb. 01, 2014; 10(2);e1004120 [Pubmed: 24586182]

Quantitative Score

  • -11.9046 [Confidence Score]

Throughput

  • High Throughput

Additional Notes

  • Conditions: rich growth medium
  • Genetic interactions were considered significant if they had an S-score >= 3.08809 for positive interactions (alleviating interactions) and S-score <= -3.38787 for negative interactions (aggravating interactions).

Curated By

  • BioGRID