BAIT

SRB5

MED18, L000002052, YGR104C

Subunit of the RNA polymerase II mediator complex; associates with core polymerase subunits to form the RNA polymerase II holoenzyme; essential for transcriptional regulation; required for proper termination of transcription for some genes; involved in telomere maintenance

GO Process (4)

GO Function (3)

GO Component (1)

Gene Ontology Biological Process

RNA polymerase II transcriptional preinitiation complex assembly [IDA]

positive regulation of transcription from RNA polymerase II promoter [IDA]

termination of RNA polymerase II transcription [IMP]

transcriptional open complex formation at RNA polymerase II promoter [IMP]

Gene Ontology Molecular Function

RNA polymerase II transcription factor recruiting transcription factor activity [IMP]
TBP-class protein binding RNA polymerase II transcription factor activity involved in preinitiation complex assembly [IDA]
core RNA polymerase II binding transcription factor activity [IDA, IGI]

Gene Ontology Cellular Component

core mediator complex [IDA]

SGD Alliance of Genome Resources Entrez Gene RefSeq UniprotKB

Saccharomyces cerevisiae (S288c)

PREY

RPB4

CTF15, DNA-directed RNA polymerase II subunit RPB4, B32, L000001678, YJL140W

RNA polymerase II subunit B32; forms dissociable heterodimer with Rpb7p; Rpb4/7 dissociates from RNAPII as Ser2 CTD phosphorylation increases; Rpb4/7 regulates cellular lifespan via mRNA decay process; involved in recruitment of 3'-end processing factors to transcribing RNAPII complex, export of mRNA to cytoplasm under stress conditions; also involved in translation initiation

GO Process (6)

GO Function (4)

GO Component (4)

Gene Ontology Biological Process

mRNA export from nucleus in response to heat stress [IMP]

nuclear-transcribed mRNA catabolic process, deadenylation-dependent decay [IMP]

positive regulation of translational initiation [IMP]

recruitment of 3'-end processing factors to RNA polymerase II holoenzyme complex [IMP]

transcription from RNA polymerase II promoter [IMP]

transcription initiation from RNA polymerase II promoter [IDA]

Gene Ontology Molecular Function

RNA-directed RNA polymerase activity [IDA]
single-stranded DNA binding [IDA]
single-stranded RNA binding [IDA]
translation initiation factor binding [IPI]

Gene Ontology Cellular Component

DNA-directed RNA polymerase II, core complex [IDA]

cytoplasm [IDA]

cytoplasmic mRNA processing body [IDA]

nucleus [IDA]

SGD Alliance of Genome Resources Entrez Gene RefSeq UniprotKB

Saccharomyces cerevisiae (S288c)

Affinity Capture-MS

An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.

Publication

Yeast nuclear extract contains two major forms of RNA polymerase II mediator complexes.

Liu Y, Ranish JA, Aebersold R, Hahn S

The yeast Mediator complex is required for transcription by RNA polymerase II (pol II) in vivo and in vitro. This complex of over 20 polypeptides associates with pol II and is recruited to transcription complexes at promoters. Previous isolations of yeast Mediator-containing complexes in different laboratories have identified several distinct complexes. To identify the major forms of Mediator in yeast, ... [more]

J. Biol. Chem. Mar. 09, 2001; 276(10);7169-75 [Pubmed: 11383511]

Throughput

Low Throughput

Related interactions

Interaction	Experimental Evidence Code	Dataset	Throughput	Score	Curated By	Notes
SRB5 RPB4	Synthetic Lethality Synthetic Lethality A genetic interaction is inferred when mutations or deletions in separate genes, each of which alone causes a minimal phenotype, result in lethality when combined in the same cell under a given condition.	Cai G (2010)	Low	-	BioGRID	428547

Curated By

BioGRID

Interaction Summary

SRB5

Gene Ontology Biological Process

Gene Ontology Molecular Function

RNA polymerase II transcription factor recruiting transcription factor activity [IMP]TBP-class protein binding RNA polymerase II transcription factor activity involved in preinitiation complex assembly [IDA]core RNA polymerase II binding transcription factor activity [IDA, IGI]

Gene Ontology Cellular Component

RPB4

Gene Ontology Biological Process

Gene Ontology Molecular Function

RNA-directed RNA polymerase activity [IDA]single-stranded DNA binding [IDA]single-stranded RNA binding [IDA]translation initiation factor binding [IPI]

Gene Ontology Cellular Component

Affinity Capture-MS

Publication

Yeast nuclear extract contains two major forms of RNA polymerase II mediator complexes.

Throughput

Related interactions

Curated By

RNA polymerase II transcription factor recruiting transcription factor activity [IMP]
TBP-class protein binding RNA polymerase II transcription factor activity involved in preinitiation complex assembly [IDA]
core RNA polymerase II binding transcription factor activity [IDA, IGI]

RNA-directed RNA polymerase activity [IDA]
single-stranded DNA binding [IDA]
single-stranded RNA binding [IDA]
translation initiation factor binding [IPI]