An interaction is inferred between proteins in vitro. This can include proteins in recombinant form or proteins isolated directly from cells with recombinant or purified bait. For example, GST pull-down assays where a GST-tagged protein is first isolated and then used to fish interactors from cell lysates are considered reconstituted complexes (e.g. PUBMED: 14657240, Fig. 4A or PUBMED: 14761940, Fig. 5). This can also include gel-shifts, surface plasmon resonance, isothermal titration calorimetry (ITC) and bio-layer interferometry (BLI) experiments. The bait-hit directionality may not be clear for 2 interacting proteins. In these cases the directionality is up to the discretion of the curator.

Publication

Leukocyte adhesion to vascular endothelium induces E-selectin linkage to the actin cytoskeleton.

Yoshida M, Westlin WF, Wang N, Ingber DE, Rosenzweig A, Resnick N, Gimbrone MA

We have examined functions of the cytoplasmic domain of E-selectin, an inducible endothelial transmembrane protein, especially its ability to associate with the cytoskeleton during leukocyte adhesion. Confocal microscopy of interleukin-1 beta (IL-1 beta)-activated human umbilical vein endothelial cells (HUVEC) visualized clustering of E-selectin molecules in the vicinity of leukocyte-endothelial cell attachment sites. A detergent based extraction and Western blotting procedure ... [more]

J. Cell Biol. Apr. 01, 1996; 133(2);445-55 [Pubmed: 8609175]

Throughput

Low Throughput

Curated By

BioGRID

Interaction Summary

SELE

Gene Ontology Biological Process

Gene Ontology Molecular Function

oligosaccharide binding [IDA]phospholipase binding [IDA]protein binding [IPI]sialic acid binding [IDA]transmembrane signaling receptor activity [IMP]

Gene Ontology Cellular Component

ACTA2