RPH3A
Gene Ontology Molecular Function
Gene Ontology Cellular Component
RAB27A
Gene Ontology Biological Process
- GTP catabolic process [IDA]
- Rab protein signal transduction [IBA]
- antigen processing and presentation [ISO]
- blood coagulation [IMP]
- complement-dependent cytotoxicity [ISO]
- cytotoxic T cell degranulation [IMP]
- exocytosis [ISO]
- exosomal secretion [ISO]
- melanocyte differentiation [IMP]
- melanosome localization [IMP, ISO]
- melanosome transport [IMP]
- multivesicular body organization [ISO]
- multivesicular body sorting pathway [ISO]
- natural killer cell degranulation [IMP]
- pigment granule localization [IMP]
- pigment granule transport [IMP]
- pigmentation [IMP]
- positive regulation of constitutive secretory pathway [ISO]
- positive regulation of exocytosis [IBA, ISO]
- positive regulation of gene expression [ISO]
- positive regulation of phagocytosis [ISO]
- positive regulation of reactive oxygen species biosynthetic process [ISO]
- positive regulation of regulated secretory pathway [ISO]
- protein secretion [IBA]
- protein targeting [IMP]
- vesicle docking involved in exocytosis [IBA]
- vesicle-mediated transport [IMP]
Gene Ontology Molecular Function
Gene Ontology Cellular Component
- Golgi apparatus [IDA]
- Weibel-Palade body [ISO]
- apical plasma membrane [ISO]
- cell [IBA]
- dendrite [ISO]
- exocytic vesicle [ISO]
- extracellular vesicular exosome [ISO]
- intracellular [IBA, ISS]
- late endosome [ISO]
- lysosome [ISO]
- melanosome [IDA, ISO]
- multivesicular body membrane [ISO]
- photoreceptor outer segment [IDA]
- secretory granule [IMP, ISO]
- secretory granule membrane [ISO]
Affinity Capture-Western
An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins.
Publication
Distinct Rab binding specificity of Rim1, Rim2, rabphilin, and Noc2. Identification of a critical determinant of Rab3A/Rab27A recognition by Rim2.
Rabphilin, Rim, and Noc2 have generally been believed to be the Rab3 isoform (Rab3A/B/C/D)-specific effectors that regulate secretory vesicle exocytosis in neurons and in some endocrine cells. The results of recent genetic analysis of rabphilin knock-out animals, however, strongly refute this notion, because there are no obvious genetic interactions between Rab3 and rabphilin in nematoda (Staunton, J., Ganetzky, B., and ... [more]
Throughput
- Low Throughput
Additional Notes
- Figure 2
- Figure 5
Curated By
- BioGRID