An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins.

Publication

Promyogenic members of the Ig and cadherin families associate to positively regulate differentiation.

Kang JS, Feinleib JL, Knox S, Ketteringham MA, Krauss RS

Determination and differentiation of cells in the skeletal muscle lineage is positively regulated by cell-cell contact. Cell-surface proteins proposed to mediate this effect include both classical cadherins and Ig superfamily members; potential interactions between the promyogenic activities of these classes of protein, however, are unknown. We show here that CDO and BOC, two promyogenic Ig superfamily members that bind to ... [more]

Proc. Natl. Acad. Sci. U.S.A. Apr. 01, 2003; 100(7);3989-94 [Pubmed: 12634428]

Throughput

Low Throughput

Curated By

BioGRID

Interaction Summary

BOC

Gene Ontology Biological Process

Gene Ontology Molecular Function

protein binding [IPI]

Gene Ontology Cellular Component