BAIT

GNAQ

CMC1, G-ALPHA-q, GAQ, SWS, RP11-494N1.1
guanine nucleotide binding protein (G protein), q polypeptide
Homo sapiens
PREY

RIC8B

RIC8, hSyn
RIC8 guanine nucleotide exchange factor B
Homo sapiens

Two-hybrid

Bait protein expressed as a DNA binding domain (DBD) fusion and prey expressed as a transcriptional activation domain (TAD) fusion and interaction measured by reporter gene activation.

Publication

Human brain synembryn interacts with Gsalpha and Gqalpha and is translocated to the plasma membrane in response to isoproterenol and carbachol.

Klattenhoff C, Montecino M, Soto X, Guzman L, Romo X, Garcia MA, Mellstrom B, Naranjo JR, Hinrichs MV, Olate J

Heterotrimeric G-proteins transduce signals from heptahelical transmembrane receptors to different effector systems, regulating diverse complex intracellular pathways and functions. In brain, facilitation of depolarization-induced neurotransmitter release for synaptic transmission is mediated by Gsalpha and Gqalpha. To identify effectors for Galpha-proteins, we performed a yeast two-hybrid screening of a human brain cDNA library, using the human Galphas protein as a bait. ... [more]

J. Cell. Physiol. May. 01, 2003; 195(2);151-7 [Pubmed: 12652642]

Throughput

  • Low Throughput

Related interactions

InteractionExperimental Evidence CodeDatasetThroughputScoreCurated ByNotes
RIC8B GNAQ
Reconstituted Complex
Reconstituted Complex

An interaction is detected between purified proteins in vitro.

Low-BioGRID
-

Curated By

  • BioGRID