BAIT

TCF3

E2A, E47, ITF1, TCF-3, VDIR, bHLHb21
transcription factor 3
GO Process (15)
GO Function (17)
GO Component (7)
Homo sapiens
PREY

BHLHE40

BHLHB2, DEC1, HLHB2, SHARP-2, STRA13, Stra14
basic helix-loop-helix family, member e40
Homo sapiens

Two-hybrid

Bait protein expressed as a DNA binding domain (DBD) fusion and prey expressed as a transcriptional activation domain (TAD) fusion and interaction measured by reporter gene activation.

Publication

Identification of interaction partners for the basic-helix-loop-helix protein E47.

Dear TN, Hainzl T, Follo M, Nehls M, Wilmore H, Matena K, Boehm T

Helix-loop-helix proteins constitute a family of transcription factors with the potential to form homo- and hetero-dimers mediated by the helix-loop-helix domain. Oncogenic mutations in such genes can disrupt the equilibrium of protein-protein interactions in the affected cell. In order to assess the biological consequences of such mutations, the full complement of interacting proteins must be known. To identify proteins interacting ... [more]

Oncogene Feb. 27, 1997; 14(8);891-8 [Pubmed: 9050988]

Throughput

  • Low Throughput

Related interactions

InteractionExperimental Evidence CodeDatasetThroughputScoreCurated ByNotes
TCF3 BHLHE40
Affinity Capture-Western
Affinity Capture-Western

An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins.

Low-BioGRID
-

Curated By

  • BioGRID