HOMER1
Gene Ontology Biological Process
Gene Ontology Molecular Function
Gene Ontology Cellular Component
GRM5
Gene Ontology Biological Process
- G-protein coupled glutamate receptor signaling pathway [IMP, ISO, ISS]
- activation of MAPKKK activity [IDA]
- cognition [ISO]
- desensitization of G-protein coupled receptor protein signaling pathway [IDA]
- learning [ISO]
- locomotory behavior [ISO]
- negative regulation of locomotion [IDA]
- phosphatidylinositol-mediated signaling [TAS]
- phospholipase C-activating G-protein coupled glutamate receptor signaling pathway [TAS]
- positive regulation of cytosolic calcium ion concentration involved in phospholipase C-activating G-protein coupled signaling pathway [TAS]
- positive regulation of long-term neuronal synaptic plasticity [IDA]
- protein kinase C-activating G-protein coupled receptor signaling pathway [IDA, TAS]
- protein phosphorylation [IDA]
- regulation of long-term neuronal synaptic plasticity [ISO]
- regulation of synaptic transmission, glutamatergic [IBA]
- regulation of transcription, DNA-templated [IDA]
- synaptic transmission [IMP]
Gene Ontology Molecular Function
Gene Ontology Cellular Component
Reconstituted Complex
An interaction is inferred between proteins in vitro. This can include proteins in recombinant form or proteins isolated directly from cells with recombinant or purified bait. For example, GST pull-down assays where a GST-tagged protein is first isolated and then used to fish interactors from cell lysates are considered reconstituted complexes (e.g. PUBMED: 14657240, Fig. 4A or PUBMED: 14761940, Fig. 5). This can also include gel-shifts, surface plasmon resonance, isothermal titration calorimetry (ITC) and bio-layer interferometry (BLI) experiments. The bait-hit directionality may not be clear for 2 interacting proteins. In these cases the directionality is up to the discretion of the curator.
Publication
Homer: a protein that selectively binds metabotropic glutamate receptors.
Spatial localization and clustering of membrane proteins is critical to neuronal development and synaptic plasticity. Recent studies have identified a family of proteins, the PDZ proteins, that contain modular PDZ domains and interact with synaptic ionotropic glutamate receptors and ion channels. PDZ proteins are thought to have a role in defining the cellular distribution of the proteins that interact with ... [more]
Throughput
- Low Throughput
Related interactions
| Interaction | Experimental Evidence Code | Dataset | Throughput | Score | Curated By | Notes |
|---|---|---|---|---|---|---|
| HOMER1 GRM5 | Affinity Capture-Western Affinity Capture-Western An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins. | Low | - | BioGRID | - | |
| HOMER1 GRM5 | Two-hybrid Two-hybrid Bait protein expressed as a DNA binding domain (DBD) fusion and prey expressed as a transcriptional activation domain (TAD) fusion and interaction measured by reporter gene activation. | Low | - | BioGRID | - |
Curated By
- BioGRID