BAIT

GRB2

ASH, EGFRBP-GRB2, Grb3-3, MST084, MSTP084, NCKAP2
growth factor receptor-bound protein 2
Homo sapiens

Reconstituted Complex

An interaction is inferred between proteins in vitro. This can include proteins in recombinant form or proteins isolated directly from cells with recombinant or purified bait. For example, GST pull-down assays where a GST-tagged protein is first isolated and then used to fish interactors from cell lysates are considered reconstituted complexes (e.g. PUBMED: 14657240, Fig. 4A or PUBMED: 14761940, Fig. 5). This can also include gel-shifts, surface plasmon resonance, isothermal titration calorimetry (ITC) and bio-layer interferometry (BLI) experiments. The bait-hit directionality may not be clear for 2 interacting proteins. In these cases the directionality is up to the discretion of the curator.

Publication

Crystal structure of the amphiphysin-2 SH3 domain and its role in the prevention of dynamin ring formation.

Owen DJ, Wigge P, Vallis Y, Moore JD, Evans PR, McMahon HT

The amphiphysins are brain-enriched proteins, implicated in clathrin-mediated endocytosis, that interact with dynamin through their SH3 domains. To elucidate the nature of this interaction, we have solved the crystal structure of the amphiphysin-2 (Amph2) SH3 domain to 2.2 A. The structure possesses several notable features, including an extensive patch of negative electrostatic potential covering a large portion of its dynamin ... [more]

EMBO J. Sep. 15, 1998; 17(18);5273-85 [Pubmed: 9736607]

Throughput

  • Low Throughput

Curated By

  • BioGRID