An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins.

Publication

Identification of an evolutionarily conserved heterotrimeric protein complex involved in protein targeting.

Borg JP, Straight SW, Kaech SM, de Taddeo-Borg M, Kroon DE, Karnak D, Turner RS, Kim SK, Margolis B

In Caenorhabditis elegans, lin-2, lin-7, and lin-10 genetically interact to control the trafficking of the Let-23 growth factor receptor to the basolateral surface of body epithelia. The human homologue of the lin-10 gene has recently been identified as a member of the X11 gene family. The X11 proteins contain one phosphotyrosine binding (PTB) and two PSD-95.Dlg.ZO-1 (PDZ) domains as well ... [more]

J. Biol. Chem. Nov. 27, 1998; 273(48);31633-6 [Pubmed: 9822620]

Throughput

Low Throughput

Additional Notes

Figure 4

Related interactions

Interaction	Experimental Evidence Code	Dataset	Throughput	Score	Curated By	Notes
LIN7C APBA1	Affinity Capture-MS Affinity Capture-MS An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.	Olsen O (2005)	Low	-	BioGRID	-
LIN7C APBA1	Affinity Capture-Western Affinity Capture-Western An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins.	Olsen O (2005)	Low	-	BioGRID	-
APBA1 LIN7C	Co-fractionation Co-fractionation Interaction inferred from the presence of two or more protein subunits in a partially purified protein preparation. If co-fractionation is demonstrated between 3 or more proteins, then add them as a complex.	Pourhaghighi R (2020)	High	0.938	BioGRID	2670507

Curated By

BioGRID

Interaction Summary

LIN7C

Gene Ontology Biological Process

Gene Ontology Molecular Function

L27 domain binding [ISO]PDZ domain binding [ISO]cytoskeletal protein binding [ISO]protein binding [IPI]protein domain specific binding [ISO]

Gene Ontology Cellular Component

APBA1

Gene Ontology Biological Process

Gene Ontology Molecular Function

PDZ domain binding [ISO]beta-amyloid binding [ISO]phosphatidylinositol-4,5-bisphosphate binding [ISO]protein binding [IPI]protein complex binding [ISO]