BAIT

LSM1

SPB8, L000004427, YJL124C

Lsm (Like Sm) protein; forms heteroheptameric complex (with Lsm2p, Lsm3p, Lsm4p, Lsm5p, Lsm6p, and Lsm7p) involved in degradation of cytoplasmic mRNAs; also enters the nucleus and positively regulates transcription initiation; unlike most Sm-like proteins, Lsm1p requires both its SM-domain and C-terminal domain for RNA-binding; binds to mRNAs under glucose starvation, most often in the 3' UTR; forms cytoplasmic foci upon DNA replication stress

GO Process (2)

GO Function (3)

GO Component (4)

Gene Ontology Biological Process

deadenylation-dependent decapping of nuclear-transcribed mRNA [IGI, IMP, IPI]

nuclear-transcribed mRNA catabolic process, deadenylation-dependent decay [IGI, IMP]

Gene Ontology Molecular Function

RNA cap binding [IGI, IMP, IPI]
chromatin binding [IDA]
mRNA binding [IDA]

Gene Ontology Cellular Component

cytoplasm [IDA]

cytoplasmic mRNA processing body [IDA, IMP]

mRNA cap binding complex [IPI]

nucleus [IDA]

SGD Alliance of Genome Resources Entrez Gene RefSeq UniprotKB

Saccharomyces cerevisiae (S288c)

PREY

SLX5

HEX3, ULS2, SUMO-targeted ubiquitin ligase complex subunit SLX5, L000000768, YDL013W

Subunit of the Slx5-Slx8 SUMO-targeted ubiquitin ligase (STUbL) complex; stimulated by SUMO-modified substrates; contains a RING domain and two SIM motifs; forms SUMO-dependent nuclear foci, including DNA repair centers; associates with the centromere; null mutants are aneuploid, have a metaphase delay, and spindle defects including: mispositioned spindles, fish hook spindles, and aberrant spindle kinetics; required for maintenance of genome integrity like human ortholog RNF4

UPS Project

GO Process (4)

GO Function (2)

GO Component (3)

Gene Ontology Biological Process

cellular response to DNA damage stimulus [IGI, IMP]

protein sumoylation [IGI, IMP]

protein ubiquitination [IDA]

telomere maintenance [IMP]

Gene Ontology Molecular Function

SUMO binding [IDA, IPI]
ubiquitin-protein transferase activity [IDA]

Gene Ontology Cellular Component

SUMO-targeted ubiquitin ligase complex [IDA]

chromosome, centromeric region [IDA]

nucleus [IDA]

SGD Alliance of Genome Resources Entrez Gene RefSeq UniprotKB

Saccharomyces cerevisiae (S288c)

Two-hybrid

Bait protein expressed as a DNA binding domain (DBD) fusion and prey expressed as a transcriptional activation domain (TAD) fusion and interaction measured by reporter gene activation.

Publication

Genome-wide protein interaction screens reveal functional networks involving Sm-like proteins.

Fromont-Racine M, Mayes AE, Brunet-Simon A, Rain JC, Colley A, Dix I, Decourty L, Joly N, Ricard F, Beggs JD, Legrain P

A set of seven structurally related Sm proteins forms the core of the snRNP particles containing the spliceosomal U1, U2, U4 and U5 snRNAs. A search of the genomic sequence of Saccharomyces cerevisiae has identified a number of open reading frames that potentially encode structurally similar proteins termed Lsm (Like Sm) proteins. With the aim of analysing all possible interactions ... [more]

Yeast Jun. 30, 2000; 17(2);95-110 [Pubmed: 10900456]

Throughput

High Throughput

Related interactions

Interaction	Experimental Evidence Code	Dataset	Throughput	Score	Curated By	Notes
LSM1 SLX5	Negative Genetic Negative Genetic Mutations/deletions in separate genes, each of which alone causes a minimal phenotype, but when combined in the same cell results in a more severe fitness defect or lethality under a given condition. This term is reserved for high or low throughput studies with scores.	Collins SR (2007)	High	-4.4379	BioGRID	218542
LSM1 SLX5	Negative Genetic Negative Genetic Mutations/deletions in separate genes, each of which alone causes a minimal phenotype, but when combined in the same cell results in a more severe fitness defect or lethality under a given condition. This term is reserved for high or low throughput studies with scores.	Costanzo M (2010)	High	-0.1833	BioGRID	390275
LSM1 SLX5	Negative Genetic Negative Genetic Mutations/deletions in separate genes, each of which alone causes a minimal phenotype, but when combined in the same cell results in a more severe fitness defect or lethality under a given condition. This term is reserved for high or low throughput studies with scores.	Costanzo M (2016)	High	-0.2022	BioGRID	2136665
LSM1 SLX5	Negative Genetic Negative Genetic Mutations/deletions in separate genes, each of which alone causes a minimal phenotype, but when combined in the same cell results in a more severe fitness defect or lethality under a given condition. This term is reserved for high or low throughput studies with scores.	Kuzmin E (2018)	High	-0.1226	BioGRID	2430631
LSM1 SLX5	Negative Genetic Negative Genetic Mutations/deletions in separate genes, each of which alone causes a minimal phenotype, but when combined in the same cell results in a more severe fitness defect or lethality under a given condition. This term is reserved for high or low throughput studies with scores.	Wilmes GM (2008)	High	-4.2666	BioGRID	308465
SLX5 LSM1	Synthetic Growth Defect Synthetic Growth Defect A genetic interaction is inferred when mutations in separate genes, each of which alone causes a minimal phenotype, result in a significant growth defect under a given condition when combined in the same cell.	Pan X (2006)	High	-	BioGRID	453291

Curated By

BioGRID

Interaction Summary

LSM1

Gene Ontology Biological Process

Gene Ontology Molecular Function

RNA cap binding [IGI, IMP, IPI]chromatin binding [IDA]mRNA binding [IDA]

Gene Ontology Cellular Component

SLX5

Gene Ontology Biological Process

Gene Ontology Molecular Function

SUMO binding [IDA, IPI]ubiquitin-protein transferase activity [IDA]

Gene Ontology Cellular Component

Two-hybrid

Publication

Genome-wide protein interaction screens reveal functional networks involving Sm-like proteins.

Throughput

Related interactions

Curated By

RNA cap binding [IGI, IMP, IPI]
chromatin binding [IDA]
mRNA binding [IDA]

SUMO binding [IDA, IPI]
ubiquitin-protein transferase activity [IDA]