Bait protein expressed as a DNA binding domain (DBD) fusion and prey expressed as a transcriptional activation domain (TAD) fusion and interaction measured by reporter gene activation.

Publication

Phospho-tyrosine dependent protein-protein interaction network.

Grossmann A, Benlasfer N, Birth P, Hegele A, Wachsmuth F, Apelt L, Stelzl U

Post-translational protein modifications, such as tyrosine phosphorylation, regulate protein-protein interactions (PPIs) critical for signal processing and cellular phenotypes. We extended an established yeast two-hybrid system employing human protein kinases for the analyses of phospho-tyrosine (pY)-dependent PPIs in a direct experimental, large-scale approach. We identified 292 mostly novel pY-dependent PPIs which showed high specificity with respect to kinases and interacting proteins ... [more]

Mol. Syst. Biol. Mar. 01, 2015; 11(3);794 [Pubmed: 25814554]

Throughput

High Throughput

Curated By

BioGRID

Interaction Summary

NOS1AP

Gene Ontology Biological Process

Gene Ontology Molecular Function

nitric-oxide synthase binding [ISS]

PLAGL2

Gene Ontology Biological Process

Gene Ontology Molecular Function

RNA polymerase II core promoter proximal region sequence-specific DNA binding transcription factor activity involved in positive regulation of transcription [IDA]sequence-specific DNA binding [IDA]sequence-specific DNA binding transcription factor activity [TAS]

Gene Ontology Cellular Component

Two-hybrid

Publication

Phospho-tyrosine dependent protein-protein interaction network.

Throughput

Curated By

RNA polymerase II core promoter proximal region sequence-specific DNA binding transcription factor activity involved in positive regulation of transcription [IDA]
sequence-specific DNA binding [IDA]
sequence-specific DNA binding transcription factor activity [TAS]