Two-hybrid

Bait protein expressed as a DNA binding domain (DBD) fusion and prey expressed as a transcriptional activation domain (TAD) fusion and interaction measured by reporter gene activation.

Publication

TGF-beta-induced apoptosis is mediated by the adapter protein Daxx that facilitates JNK activation.

Perlman R, Schiemann WP, Brooks MW, Lodish HF, Weinberg RA

Transforming growth factor-beta (TGF-beta) is a multifunctional growth factor that has a principal role in growth control through both its cytostatic effect on many different epithelial cell types and its ability to induce programmed cell death in a variety of other cell types. Here we have used a screen for proteins that interact physically with the cytoplasmic domain of the ... [more]

Nat. Cell Biol. Aug. 01, 2001; 3(8);708-14 [Pubmed: 11483955]

Throughput

  • Low Throughput

Additional Notes

  • Figure 1

Curated By

  • BioGRID