Two-hybrid

Bait protein expressed as a DNA binding domain (DBD) fusion and prey expressed as a transcriptional activation domain (TAD) fusion and interaction measured by reporter gene activation.

Publication

Rab40C is a novel Varp-binding protein that promotes proteasomal degradation of Varp in melanocytes.

Yatsu A, Shimada H, Ohbayashi N, Fukuda M

Varp (VPS9-ankyrin repeat protein) was originally identified as an activator of small GTPase Rab21 through its VPS9 domain, but it has subsequently been shown to function as a Rab32/38 effector through its first ANKR1 domain. Although these functions of Varp are important for melanogenesis, Varp contains a second ANKR2 domain, whose function remained completely unknown. Here we identified Rab40C, an ... [more]

Biol Open Feb. 11, 2015; 4(3);267-75 [Pubmed: 25661869]

Throughput

  • Low Throughput

Curated By

  • BioGRID