BAIT

LEMD3

man1, sane, xman1
LEM domain containing 3
GO Process (0)
GO Function (0)
GO Component (0)
Xenopus laevis
PREY

TMEM194A-B

nemp1, nemp1b, tmem194a, tmem194ab
transmembrane protein 194A
GO Process (0)
GO Function (0)
GO Component (0)
Xenopus laevis

Affinity Capture-Western

An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins.

Publication

The Inner Nuclear Membrane Protein Nemp1 Is a New Type of RanGTP-Binding Protein in Eukaryotes.

Shibano T, Mamada H, Hakuno F, Takahashi S, Taira M

The inner nuclear membrane (INM) protein Nemp1/TMEM194A has previously been suggested to be involved in eye development in Xenopus, and contains two evolutionarily conserved sequences in the transmembrane domains (TMs) and the C-terminal region, named region A and region B, respectively. To elucidate the molecular nature of Nemp1, we analyzed its interacting proteins through those conserved regions. First, we found ... [more]

PLoS ONE May. 07, 2015; 10(5);e0127271 [Pubmed: 25946333]

Throughput

  • Low Throughput

Curated By

  • BioGRID