Affinity Capture-Western

An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins.

Publication

Association of Trk neurotrophin receptors with components of the cytoplasmic dynein motor.

Yano H, Lee FS, Kong H, Chuang J, Arevalo J, Perez P, Sung C, Chao MV

Nerve growth factor (NGF) initiates its trophic effects by long-range signaling through binding, internalization, and transport of a ligand-receptor complex from the axon terminal to the cell body. However, the mechanism by which retrograde transport of NGF takes place has not been elucidated. Here we describe an interaction between the Trk receptor tyrosine kinase and a 14 kDa light chain ... [more]

J. Neurosci. Feb. 01, 2001; 21(3);RC125 [Pubmed: 11157096]

Throughput

  • Low Throughput

Curated By

  • BioGRID