BAIT

PTEN

10q23del, BZS, CWS1, DEC, GLM2, MHAM, MMAC1, PTEN1, TEP1
phosphatase and tensin homolog
GO Process (64)
GO Function (11)
GO Component (10)

Gene Ontology Biological Process

Homo sapiens
PREY

TUBB3

CDCBM, CDCBM1, CFEOM3, CFEOM3A, FEOM3, TUBB4, beta-4
tubulin, beta 3 class III
GO Process (4)
GO Function (1)
GO Component (4)
Homo sapiens

Affinity Capture-MS

An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.

Publication

WWP2 is an E3 ubiquitin ligase for PTEN.

Maddika S, Kavela S, Rani N, Palicharla VR, Pokorny JL, Sarkaria JN, Chen J

PTEN, a lipid phosphatase, is one of the most frequently mutated tumour suppressors in human cancer. Several recent studies have highlighted the importance of ubiquitylation in regulating PTEN tumour-suppressor function, but the enzymatic machinery required for PTEN ubiquitylation is not clear. In this study, by using a tandem affinity-purification approach, we have identified WWP2 (also known as atrophin-1-interacting protein 2, ... [more]

Nat. Cell Biol. Jun. 01, 2011; 13(6);728-33 [Pubmed: 21532586]

Throughput

  • High Throughput

Curated By

  • BioGRID