BAIT

RALGDS

RGDS, RGF, RalGEF, RP11-326L24.1
ral guanine nucleotide dissociation stimulator
GO Process (3)
GO Function (2)
GO Component (1)

Gene Ontology Biological Process

Gene Ontology Molecular Function

Gene Ontology Cellular Component

Homo sapiens
PREY

CNKSR1

CNK, CNK1
connector enhancer of kinase suppressor of Ras 1
GO Process (3)
GO Function (1)
GO Component (3)

Gene Ontology Biological Process

Gene Ontology Molecular Function

Gene Ontology Cellular Component

Homo sapiens

Affinity Capture-Western

An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins.

Publication

Human CNK1 acts as a scaffold protein, linking Rho and Ras signal transduction pathways.

Jaffe AB, Aspenstroem P, Hall A

Rho family GTPases act as molecular switches to control a variety of cellular responses, including cytoskeletal rearrangements, changes in gene expression, and cell transformation. In the active, GTP-bound state, Rho interacts with an ever-growing number of effector molecules, which promote distinct biochemical pathways. Here, we describe the isolation of hCNK1, the human homologue of Drosophila connector enhancer of ksr, as ... [more]

Mol. Cell. Biol. Feb. 01, 2004; 24(4);1736-46 [Pubmed: 14749388]

Throughput

  • Low Throughput

Additional Notes

  • Figure 7

Related interactions

InteractionExperimental Evidence CodeDatasetThroughputScoreCurated ByNotes
CNKSR1 RALGDS
Affinity Capture-Western
Affinity Capture-Western

An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins.

Low-BioGRID
252424

Curated By

  • BioGRID