PREY

HAPLN1

BB099155, CLP, Crtl1, Crtl1l, LP, LP-1
hyaluronan and proteoglycan link protein 1
GO Process (3)
GO Function (2)
GO Component (2)
Mus musculus

Affinity Capture-Western

An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins.

Publication

Distinct interaction of versican/PG-M with hyaluronan and link protein.

Matsumoto K, Shionyu M, Go M, Shimizu K, Shinomura T, Kimata K, Watanabe H

The proteoglycan aggregate is the major structural component of the cartilage matrix, comprising hyaluronan (HA), link protein (LP), and a large chondroitin sulfate (CS) proteoglycan, aggrecan. Here, we found that another member of aggrecan family, versican, biochemically binds to both HA and LP. Functional analyses of recombinant looped domains (subdomains) A, B, and B' of the N-terminal G1 domain revealed ... [more]

J. Biol. Chem. Oct. 17, 2003; 278(42);41205-12 [Pubmed: 12888576]

Throughput

  • Low Throughput

Curated By

  • BioGRID