FLW
Gene Ontology Biological Process
- cell adhesion [NAS]
- chromosome segregation [IMP]
- female germline ring canal formation [IMP]
- imaginal disc-derived wing morphogenesis [IMP]
- instar larval development [NAS]
- mesoderm development [NAS]
- muscle attachment [NAS]
- negative regulation of JNK cascade [IGI]
- oocyte nucleus migration involved in oocyte dorsal/ventral axis specification [IMP]
- ovarian nurse cell to oocyte transport [IMP]
- protein dephosphorylation [ISS, NAS]
- regulation of actomyosin contractile ring contraction [IMP]
- striated muscle tissue development [IMP]
Gene Ontology Molecular Function
Gene Ontology Cellular Component
PITSLRE
Gene Ontology Biological Process
Gene Ontology Molecular Function
Affinity Capture-MS
An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.
Publication
In vivo analysis of proteomes and interactomes using Parallel Affinity Capture (iPAC) coupled to mass spectrometry.
Affinity purification coupled to mass spectrometry provides a reliable method for identifying proteins and their binding partners. In this study we have used Drosophila melanogaster proteins triple tagged with Flag, Strep II, and Yellow fluorescent protein in vivo within affinity pull-down experiments and isolated these proteins in their native complexes from embryos. We describe a pipeline for determining interactomes by ... [more]
Throughput
- High Throughput
Related interactions
| Interaction | Experimental Evidence Code | Dataset | Throughput | Score | Curated By | Notes |
|---|---|---|---|---|---|---|
| FLW PITSLRE | Affinity Capture-MS Affinity Capture-MS An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods. | High | - | BioGRID | - |
Curated By
- BioGRID